Surfactant free synthesis of cationic nano-vesicles: A safe triple drug loaded vehicle for the topical treatment of cutaneous leishmaniasis.

Abstract

The basic aim of the study was to develop and evaluate the triple drug loaded cationic nano-vesicles (cNVs), where miltefosine was used as a replacement of surfactant (apart from its anti-leishmanial role), in addition to meglumine antimoniate (MAM) and imiquimod (Imq), as a combination therapy for the topical treatment of cutaneous leishmaniasis (CL). The optimized formulation was nano-sized (86.2 ± 2.7 nm) with high entrapment efficiency (63.8 ± 2.1% (MAM) and 81.4 ± 2.3% (Imq)). In-vivo skin irritation assay showed reduced irritation potential and a decrease in the cytotoxicity of cNVs as compared to conventional NVs (having sodium deoxycholate as a surfactant). A synergistic interaction between drugs was observed against intracellular amastigotes, whereas the in-vivo antileishmanial study presented a significant reduction in the parasitic burden. The results suggested the potential of surfactant free, triple drug loaded cNVs as an efficient vehicle for the safe topical treatment of CL.

• Authors: Sidra Khalid, Saad Salman, Kashif Iqbal, Fiza Ur Rehman, Iffat Ullah, Abhay R Satoskar, Gul Majid Khan, M Junaid Dar
• Journal: Nanomedicine : nanotechnology, biology, and medicine (Nanomedicine) Vol. 40 Pg. 102490 (02 2022) ISSN: 1549-9642 [Electronic] United States
• PMID: 34748957 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• Antiprotozoal Agents
• Surface-Active Agents

Topics

• Administration, Topical
• Antiprotozoal Agents (pharmacology)
• Humans
• Leishmania
• Leishmaniasis, Cutaneous (drug therapy)
• Surface-Active Agents