Bladder cancer is a common malignant
tumor with a high recurrence rate and mortality, while the detailed mechanisms for
bladder cancer progression and
metastasis are unknown. Recently, long non-coding RNAs (lncRNAs) have been reported to be involved in the development of
cancers. In this study, we aim to investigate the role of
lncRNA LINC00355 in
bladder cancer progression and
metastasis. The association between LINC00355 and the prognosis of
bladder cancer patients was determined by Kaplan-Meier survival analysis. Cell migration and invasion ability were detected using the Transwell migration and invasion assay. The relationships of LINC00355, miR-424-5p, and High Mobility Group AT-Hook 2 (HMGA2) were verified through the
luciferase assay and
RNA pull-down assay. Xenograft
tumor was established to evaluate
tumor lung
metastasis in vivo. qRT-PCR and western blot were used to detect gene expression. LINC00355 was upregulated in
bladder cancer patients, especially in patients with higher TNM stage. Elevated LINC00355 was correlated with the poor prognosis of
bladder cancer patients. Besides, overexpressed LINC00355 promoted migration, invasion, and epithelial-mesenchymal transition (EMT) ability of
bladder cancer cells. Contrarily, decreased LINC00355 suppressed migration, invasion, and EMT ability of
bladder cancer cells, and lung
metastasis of xenograft
tumors. Furthermore, LINC00355 could regulate HMGA2 expression by acting as a sponge for miR-424-5p. Overexpression of HMGA2 induced EMT of
bladder cancer cells. Additionally, LINC00355 regulated the migration, invasion, and EMT ability of
bladder cancer cells through modulating HMGA2 expression via sponging miR-424-5p. LINC00355 promoted migration, invasion, and EMT ability of
bladder cancer through elevating HMGA2 expression via acting as a sponge for miR-424-5p.