Development of a DELFIA method to detect oncofetal antigen ROR1-positive exosomes.

Abstract

Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is highly expressed in a wide variety of hematological and solid cancers, but is low or absent in adult tissues. Here, we show that ROR1 is released with exosomes from ROR1-positive cancer cells. We also developed a simple dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) to detect cancer-derived ROR1-positive exosomes, which are captured by two anti-ROR1 antibodies and detected by the fluorescence of free chelating europium. This new DELFIA method can detect cancer-derived ROR1-positive exosomes in the cell supernatant and serum with a wide range and rapidly compared with the conventional Western blot assay. This method may be useful as a companion diagnostics for ROR1-positive cancers.

• Authors: Hina Daikuzono, Masaya Yamazaki, Yoshifumi Sato, Takashi Takahashi, Kazuya Yamagata
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 578 Pg. 170-176 (11 12 2021) ISSN: 1090-2104 [Electronic] United States
• PMID: 34597914 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• oncofetal antigens
• ROR1 protein, human
• Receptor Tyrosine Kinase-like Orphan Receptors

Topics

• Animals
• Antigens, Neoplasm (metabolism)
• Biomarkers, Tumor (blood, metabolism)
• Cell Line, Tumor
• Disease Models, Animal
• Exosomes (metabolism, pathology)
• Female
• Humans
• Immunoassay (methods)
• Mice
• Mice, Inbred BALB C
• Neoplasms (blood, metabolism, pathology)
• Receptor Tyrosine Kinase-like Orphan Receptors (metabolism)