Abstract |
Programmed cell death-1 (PD-1) signaling downregulates the T-cell response, promoting an exhausted state in tumor-infiltrating T cells, through mostly unveiled molecular mechanisms. Dynamin-related protein-1 (Drp1)-dependent mitochondrial fission plays a crucial role in sustaining T-cell motility, proliferation, survival, and glycolytic engagement. Interestingly, such processes are exactly those inhibited by PD-1 in tumor-infiltrating T cells. Here, we show that PD-1pos CD8+ T cells infiltrating an MC38 (murine adenocarcinoma)-derived murine tumor mass have a downregulated Drp1 activity and more elongated mitochondria compared with PD-1neg counterparts. Also, PD-1pos lymphocytic elements infiltrating a human colon cancer rarely express active Drp1. Mechanistically, PD-1 signaling directly prevents mitochondrial fragmentation following T-cell stimulation by downregulating Drp1 phosphorylation on Ser616, via regulation of the ERK1/2 and mTOR pathways. In addition, downregulation of Drp1 activity in tumor-infiltrating PD-1pos CD8+ T cells seems to be a mechanism exploited by PD-1 signaling to reduce motility and proliferation of these cells. Overall, our data indicate that the modulation of Drp1 activity in tumor-infiltrating T cells may become a valuable target to ameliorate the anticancer immune response in future immunotherapy approaches.
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Authors | Luca Simula, Ylenia Antonucci, Giorgia Scarpelli, Valeria Cancila, Alessandra Colamatteo, Simona Manni, Biagio De Angelis, Concetta Quintarelli, Claudio Procaccini, Giuseppe Matarese, Claudio Tripodo, Silvia Campello |
Journal | Molecular oncology
(Mol Oncol)
Vol. 16
Issue 1
Pg. 188-205
(01 2022)
ISSN: 1878-0261 [Electronic] United States |
PMID | 34535949
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. |
Chemical References |
- Pdcd1 protein, mouse
- Programmed Cell Death 1 Receptor
- Dnm1l protein, mouse
- Dynamins
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Topics |
- Animals
- CD8-Positive T-Lymphocytes
(metabolism)
- Dynamins
(immunology, metabolism)
- Humans
- Mice
- Mitochondria
(metabolism)
- Mitochondrial Dynamics
- Programmed Cell Death 1 Receptor
(immunology, metabolism)
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