Photosensitizing nanoclays for efficient cell uptake and in vitro photodynamic therapy.

Abstract

In this study a straightforward strategy to deliver photosensitizing molecules into cancer cells by using Laponite RD® (LAP) nanodisks as carrier is presented. We report the application of LAP functionalized with a highly hydrophobic Silicon phthalocyanine photosensitizer (SiPc) for efficient cell uptake and photodynamic therapy (PDT) of MCF-7 breast cancer cells in vitro. This inorganic-organic hybrid nanomaterial caused a threefold increase in intracellular ROS levels and 99.7% of cancer cell inactivation after light treatment (630 nm; fluency of 108 J/cm²). With its theranostic capabilities (simultaneous fluorescence and singlet oxygen generation), uptake into cancer cells was monitored to control the photo-inactivation. The functionalized nanodisks already proved to be an efficient photo-inactivator of pathogenic Gram-(+) bacteria and were previously reported as LS10. By extending the use of LS10 to PDT of cancer cells, it is an example of a photosensitizer functionalized nanoclay with multipurpose drug characteristics, demonstrating an intriguing potential for future phototherapeutic studies and applications.

• Authors: Malte C Grüner, Ilaiáli S Leite, Natalia M Inada, Andrea S de Camargo
• Journal: Photodiagnosis and photodynamic therapy (Photodiagnosis Photodyn Ther) Vol. 35 Pg. 102384 (Sep 2021) ISSN: 1873-1597 [Electronic] Netherlands
• PMID: 34116252 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Photosensitizing Agents
• Singlet Oxygen

Topics

• Cell Line, Tumor
• Hydrophobic and Hydrophilic Interactions
• Nanostructures
• Photochemotherapy (methods)
• Photosensitizing Agents (pharmacology)
• Singlet Oxygen