Human dental pulp stem cell responses to different dental pulp capping materials.

Abstract

BACKGROUND:

Direct pulp capping is a vital pulp therapy for a pin-point dental pulp exposure. Applying a pulp capping material leads to the formation of a dentin bridge and protects pulp vitality. The aim of this study was to compare the effects of four dental materials, DyCal®, ProRoot® MTA, Biodentine™, and TheraCal™ LC in vitro.

METHODS:

Human dental pulp stem cells (hDPs) were isolated and characterized. Extraction medium was prepared from the different pulp capping materials. The hDP cytotoxicity, proliferation, and migration were examined. The odonto/osteogenic differentiation was determined by alkaline phosphatase, Von Kossa, and alizarin red s staining. Osteogenic marker gene expression was evaluated using real-time polymerase chain reaction.

RESULTS:

ProRoot® MTA and Biodentine™ generated less cytotoxicity than DyCal® and TheraCal™ LC, which were highly toxic. The hDPs proliferated when cultured with the ProRoot® MTA and Biodentine™ extraction media. The ProRoot® MTA and Biodentine™ extraction medium induced greater cell attachment and spreading. Moreover, the hDPs cultured in the ProRoot® MTA or Biodentine™ extraction medium migrated in a similar manner to those in serum-free medium, while a marked reduction in cell migration was observed in the cells cultured in DyCal® and TheraCal™ LC extraction media. Improved mineralization was detected in hDPs maintained in ProRoot® MTA or Biodentine™ extraction medium compared with those in serum-free medium.

CONCLUSION:

This study demonstrates the favorable in vitro biocompatibility and bioactive properties of ProRoot® MTA and Biodentine™ on hDPs, suggesting their superior regenerative potential compared with DyCal® and TheraCal™.

• Authors: Chawan Manaspon, Chavin Jongwannasiri, Sujin Chumprasert, Noppadol Sa-Ard-Iam, Rangsini Mahanonda, Prasit Pavasant, Thantrira Porntaveetus, Thanaphum Osathanon
• Journal: BMC oral health (BMC Oral Health) Vol. 21 Issue 1 Pg. 209 (04 26 2021) ISSN: 1472-6831 [Electronic] England
• PMID: 33902558 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aluminum Compounds
• Calcium Compounds
• Drug Combinations
• Oxides
• Pulp Capping and Pulpectomy Agents
• Silicates

Topics

• Aluminum Compounds
• Calcium Compounds
• Dental Pulp
• Dental Pulp Capping
• Drug Combinations
• Humans
• Osteogenesis
• Oxides (pharmacology)
• Pulp Capping and Pulpectomy Agents
• Silicates
• Stem Cells