Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling.

Abstract

BACKGROUND/AIM:

Lewis y is expressed in oral squamous cell carcinoma (OSCC) cells and tumors. Previously, we reported that Lewis y was not expressed in invasion areas, and attenuation of proliferation and invasion in OSCC cells was caused by over-expression of Lewis y. However, the roles of Lewis y in the attenuation of malignant properties have not been clarified. In this study, we investigated the roles of Lewis y in OSCC.

MATERIALS AND METHODS:

The levels of Lewis y on EGFR and the phosphorylation levels of EGFR in OSCC cells were analyzed by immunoprecipitation and western blot. EGFR cross-linking and binding kinetics of EGF were performed.

RESULTS:

Upon EGF stimulation, phosphorylation and dimer formation of EGFR were more prominent in Lewis y- cells. EGF binding kinetics showed reduced binding sites in Lewis y+ cells.

CONCLUSION:

Lewis y reduced EGF binding to EGFR, leading to suppression of malignant properties through suppression of EGF signaling.

• Authors: Hiroshi Hotta, Kazunori Hamamura, Hidenobu Shibuya, Yuhsuke Ohmi, Keiko Furukawa, Koichi Furukawa
• Journal: Anticancer research (Anticancer Res) Vol. 41 Issue 4 Pg. 1821-1830 (Apr 2021) ISSN: 1791-7530 [Electronic] Greece
• PMID: 33813387 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Lewis Blood Group Antigens
• Lewis Y antigen
• Epidermal Growth Factor
• EGFR protein, human
• ErbB Receptors

Topics

• Binding Sites
• Cell Adhesion
• Cell Line, Tumor
• Cell Movement
• Cell Shape
• Epidermal Growth Factor (metabolism)
• ErbB Receptors (metabolism)
• Humans
• Kinetics
• Lewis Blood Group Antigens (metabolism)
• Mouth Neoplasms (metabolism, pathology)
• Neoplasm Invasiveness
• Phosphorylation
• Protein Binding
• Signal Transduction
• Squamous Cell Carcinoma of Head and Neck (metabolism, pathology)