The
herbicide aclonifen is commonly used in agriculture.
Aclonifen is toxic to experimental animals, causing developmental abnormalities, decreased energy production for survival, and impaired organogenesis. However, no studies have reported the functional defects and toxicity caused by
aclonifen in embryonic development. We hypothesized that the mechanism underlying the toxicity of several
herbicides in various organisms involves
mitochondrial dysfunction, which subsequently promotes genotoxicity, cytotoxicity, and acute organotoxicity. In the present study, we demonstrated that
mitochondrial dysfunction during development results in decreased body length, delayed yolk sac absorption, malformed spinal cord, disrupted brain and eye formation, and the activation of apoptosis in zebrafish embryos.
Aclonifen induced oxidative stress by elevating the level of
reactive oxygen species, causing mitochondrial damage. Likewise, impaired embryonic vascularization can promote cardiovascular disorders. In this study, we characterized the toxicity of
aclonifen in a non-target organism. These findings increase our understanding of the toxicological effects of
herbicides in unexpected environments.