In this study, we found that
maspin affects the development of drug resistance in
lung adenocarcinoma. Therefore, it is important to clarify the role and mechanism of mammary
serine protease inhibitor (
maspin) in the regulation of
adenocarcinoma drug resistance in order to improve individualized clinical treatment protocols and drug resistance interventions.
METHODS: Immunohistochemical was used to detect
maspin expression in tissue chip samples of 75 patients diagnosed with
lung adenocarcinoma and treated with a taxus
chemotherapy regimen, and the correlation between
maspin, clinicopathological factors, and prognosis was analyzed. The expression of
maspin in a human
lung adenocarcinoma docetaxel-resistant cell line, SPC-A1/DTX, and its parent cells were detected by reverse transcription polymerase chain reaction (RT-PCR) and western blot assay. MTT and flow cytometry were used to detect the effects of knockdown or overexpression of
maspin on
chemotherapy sensitivity and apoptosis in
lung cancer cells.
Tumor cells were also analyzed in vivo to determine their tumorigenic ability and susceptibility to
docetaxel.
RESULTS:
Maspin is poorly expressed in
lung adenocarcinoma tissue chips that have received a taxus
chemotherapy regimen, and is also closely related to poor grading, late stage,
lymph node metastasis, and poor prognosis.
Maspin has a low expression in
drug-resistant cells, and the expression level of
maspin decreases significantly with increases in
docetaxel concentration and over time. In
drug-resistant cells, knockdown of
maspin can significantly affect the sensitivity of
drug-resistant cells to
docetaxel. In the
chemotherapy-sensitive strain SPC-A1,
maspin was mainly located in the cell nucleus, while in the
chemotherapy-resistant strain SPC-A1/DTX,
maspin was mainly located in the cytoplasm. An in vivo nude mouse xenograft model showed that an overexpression of
maspin significantly increased the inhibitory effect of
docetaxel on
tumor-bearing tissues and the apoptosis rate, and markedly reduced
tumor weight, volume, and the Ki-67-positive rate.
CONCLUSIONS: