Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis.

Abstract

Bone volume inadequacy is an emerging clinical problem impairing the feasibility and longevity of dental implants. Human bone marrow mesenchymal stem cells (HBMSCs) have been widely used in bone remodeling and regeneration. This study examined the effect of long noncoding RNAs (lncRNAs)-H19 on the human amnion-derived mesenchymal stem cells (HAMSCs)-droved osteogenesis in HBMSCs. HAMSCs and HBMSCs were isolated from abandoned amniotic membrane samples and bone marrow. The coculture system was conducted using transwells, and H19 level was measured by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). The mechanism was further verified. We here discovered that osteogenesis of HBMSCs was induced by HAMSCs, while H19 level in HAMSCs was increased during coculturing. H19 had no significant effect on the proliferative behaviors of HBMSCs, while its overexpression of H19 in HAMSCs led to the upregulated osteogenesis of HBMSCs in vivo and in vitro; whereas its knockdown reversed these effects. Mechanistically, H19 promoted miR-675 expression and contributed to the competitively bounding of miR-675 and Adenomatous polyposis coli (APC), thus significantly activating the Wnt/β-catenin pathway. The results suggested that HAMSCs promote osteogenic differentiation of HBMSCs via H19/miR-675/APC pathway, and supply a potential target for the therapeutic treatment of bone-destructive diseases.

• Authors: Xiaojie Ma, Yifeng Bian, Hua Yuan, Ning Chen, Yongchu Pan, Weina Zhou, Shiyu Gao, Xin Du, Shushu Hao, Zixin Yan, Xuan Li, Keyue Liu, Fan Xu, Yuli Wang, Yifei Du
• Journal: Aging (Aging (Albany NY)) Vol. 12 Issue 11 Pg. 10527-10543 (05 20 2020) ISSN: 1945-4589 [Electronic] United States
• PMID: 32434960 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• APC protein, human
• Adenomatous Polyposis Coli Protein
• H19 long non-coding RNA
• MIRN675 microRNA, human
• MicroRNAs
• RNA, Long Noncoding

Topics

• Adenomatous Polyposis Coli Protein (genetics)
• Amnion (cytology)
• Animals
• Bone Marrow Cells (physiology)
• Cell Differentiation (genetics)
• Cells, Cultured
• Coculture Techniques
• Dental Implantation (methods)
• Disease Models, Animal
• Female
• Humans
• Mandible (diagnostic imaging, physiology)
• Mandibular Injuries (therapy)
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells (metabolism)
• MicroRNAs (genetics, metabolism)
• Osteogenesis (genetics)
• Primary Cell Culture
• RNA, Long Noncoding (metabolism)
• Rats
• Wnt Signaling Pathway (genetics)
• X-Ray Microtomography