Modeling atrial fibrosis in vitro-Generation and characterization of a novel human atrial fibroblast cell line.

Abstract

Atrial fibrillation (AF) is regularly accompanied by cardiac fibrosis and concomitant heart failure. Due to the heterogeneous nature and complexity of fibrosis, the knowledge about the underlying mechanisms is limited, which prevents effective pharmacotherapy. A deeper understanding of cardiac fibroblasts is essential to meet this need. We previously described phenotypic and functional differences between atrial fibroblasts from patients in sinus rhythm and with AF. Herein, we established and characterized a novel human atrial fibroblast line, which displays typical fibroblast morphology and function comparable to primary cells but with improved proliferation capacity and low spontaneous myofibroblast differentiation. These traits make our model suitable for the study of fibrosis mechanisms and for drug screening aimed at developing effective antifibrotic pharmacotherapy.

• Authors: Stephan R Künzel, Johanna S E Rausch, Charlotte Schäffer, Maximilian Hoffmann, Karolina Künzel, Erik Klapproth, Theresa Kant, Natalie Herzog, Jan-Heiner Küpper, Kristina Lorenz, Svenja Dudek, Ramona Emig, Ursula Ravens, Eva A Rog-Zielinska, Rémi Peyronnet, Ali El-Armouche
• Journal: FEBS open bio (FEBS Open Bio) Vol. 10 Issue 7 Pg. 1210-1218 (07 2020) ISSN: 2211-5463 [Electronic] England
• PMID: 32421922 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

Topics

• Cell Differentiation
• Cell Proliferation
• Cells, Cultured
• Fibroblasts (metabolism, pathology)
• Fibrosis (metabolism, pathology)
• Heart Atria (metabolism, pathology)
• Humans
• Models, Biological