Identification of SARS-CoV RBD-targeting monoclonal antibodies with cross-reactive or neutralizing activity against SARS-CoV-2.

Abstract

SARS-CoV-2-caused COVID-19 cases are growing globally, calling for developing effective therapeutics to control the current pandemic. SARS-CoV-2 and SARS-CoV recognize angiotensin-converting enzyme 2 (ACE2) receptor via the receptor-binding domain (RBD). Here, we identified six SARS-CoV RBD-specific neutralizing monoclonal antibodies (nAbs) that cross-reacted with SARS-CoV-2 RBD, two of which, 18F3 and 7B11, neutralized SARS-CoV-2 infection. 18F3 recognized conserved epitopes on SARS-CoV and SARS-CoV-2 RBDs, whereas 7B11 recognized epitopes on SARS-CoV RBD not fully conserved in SARS-CoV-2 RBD. The 18F3-recognizing epitopes on RBD did not overlap with the ACE2-binding sites, whereas those recognized by 7B11 were close to the ACE2-binding sites, explaining why 7B11 could, but 18F3 could not, block SARS-CoV or SARS-CoV-2 RBD binding to ACE2 receptor. Our study provides an alternative approach to prevent SARS-CoV-2 infection using anti-SARS-CoV nAbs.

• Authors: Wanbo Tai, Xiujuan Zhang, Yuxian He, Shibo Jiang, Lanying Du
• Journal: Antiviral research (Antiviral Res) Vol. 179 Pg. 104820 (07 2020) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 32405117 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Monoclonal
• Antibodies, Neutralizing
• Antibodies, Viral
• Antigens, Viral
• Epitopes
• Spike Glycoprotein, Coronavirus
• spike glycoprotein, SARS-CoV
• Peptidyl-Dipeptidase A
• ACE2 protein, human
• Angiotensin-Converting Enzyme 2

Topics

• Amino Acid Sequence
• Angiotensin-Converting Enzyme 2
• Antibodies, Monoclonal (immunology, isolation & purification)
• Antibodies, Neutralizing (immunology)
• Antibodies, Viral (immunology)
• Antigens, Viral (chemistry, genetics, immunology)
• Betacoronavirus (genetics, immunology)
• Binding Sites
• COVID-19
• Coronavirus Infections (virology)
• Cross Reactions
• Epitopes (immunology)
• HEK293 Cells
• Humans
• Neutralization Tests
• Pandemics
• Peptidyl-Dipeptidase A (immunology)
• Pneumonia, Viral (virology)
• Severe acute respiratory syndrome-related coronavirus (genetics, immunology)
• SARS-CoV-2
• Spike Glycoprotein, Coronavirus (chemistry, genetics, immunology)