Co-existence of OXA-48 and NDM-1 in colistin resistant Pseudomonas aeruginosa ST235.

Abstract

Here, we presented 11 cases with colistin-resistant Pseudomonas aeruginosa infection and co-existence of OXA-48 and NDM-1 in the ST235 high-risk clone. The molecular analyses were performed by Sanger sequencing and RT-PCR. The eight patients (72.7%) had an invasive infection and three (27.3%) had colonization. The 30-day mortality rate was 87.5% (7/8). Three patients (37.5%, 3/8) received colistin therapy before isolation of P. aeruginosa. In the Multilocus sequence typing (MLST) analysis of 11 isolates, eight (72.7%) isolates belonged to P. aeruginosa ST235 clone. All isolates were NDM-1 positive, and nine isolates (81.8%) were found to be positive for both OXA-48 and NDM-1. Sequences of pmrAB and phoPQ revealed numerous insertions and deletions in all isolates. In 10 isolates pmrAB and phoPQ were found to be upregulated. In conclusion, the co-existence of OXA-48 and NDM-1 genes in colistin-resistant P. aeruginosa ST235 high-risk clone indicates the spread of carbapenemases in clinical isolates and highlights need of continuous surveillance for high-risk clones of P. aeruginosa.

• Authors: Cansel Vatansever, Sirin Menekse, Ozlem Dogan, Lal Sude Gucer, Berna Ozer, Onder Ergonul, Fusun Can
• Journal: Emerging microbes & infections (Emerg Microbes Infect) Vol. 9 Issue 1 Pg. 152-154 ( 2020) ISSN: 2222-1751 [Electronic] United States
• PMID: 31964275 (Publication Type: Letter)

Chemical References

• Anti-Bacterial Agents
• Bacterial Proteins
• beta-Lactamases
• beta-lactamase NDM-1
• Colistin

Topics

• Anti-Bacterial Agents (pharmacology)
• Bacterial Proteins (genetics, metabolism)
• Colistin (pharmacology)
• Drug Resistance, Bacterial
• Humans
• Microbial Sensitivity Tests
• Pseudomonas Infections (microbiology, mortality)
• Pseudomonas aeruginosa (classification, drug effects, enzymology)
• beta-Lactamases (genetics, metabolism)