It has been reported that
18F-FDG uptake is higher in hypoxic
cancer cells than in well-oxygenated cells. We demonstrated that
18F-FDG uptake in
lung cancer would be affected by high concentration
oxygen breathing. Methods. Overnight fasted
non-small-cell lung cancer A549 subcutaneous (s.c.) xenografts bearing mice (n = 10) underwent
18F-FDG micro-PET scans, animals breathed room air on day 1, and same animals breathed
carbogen (95% O2 + 5% CO2) on the subsequent day. In separated studies, autoradiography and immunohistochemical staining visualization of frozen section of A549 s.c.
tumors were applied, and to compare between
carbogen-breathing mice and those with air breathing, a combination of
18F-FDG and
hypoxia marker
pimonidazole was injected 1 h before animal sacrifice, and
18F-FDG accumulation was compared with
pimonidazole binding and
glucose transporter 1 (GLUT-1) expression. Results. PET studies revealed that
tumor 18F-FDG uptake was significantly decreased in
carbogen-breathing mice than those with air breathing (P < 0.05). Ex vivo studies confirmed that
carbogen breathing significantly decreased hypoxic fraction detected by
pimonidazole staining, referring to GLUT-1 expression, and significantly decreased
18F-FDG accumulation in
tumors. Conclusions. High concentration of O2 breathing during
18F-FDG uptake phase significantly decreases
18F-FDG uptake in
non-small-cell lung cancer A549 xenografts growing in mice.