Expression and biological functions of the CCL5-CCR5 axis in oral lichen planus.

Abstract

Oral lichen planus (OLP) is a chronic inflammatory oral mucosal disorder with T cell-mediated immunological pathogenesis. C-C motif chemokine ligand 5 (CCL5) and its receptor C-C motif chemokine receptor 5 (CCR5) play important roles in the activation and recruitment of T cells. This study sought to explore the expression and biological functions of the CCL5-CCR5 axis in OLP. We examined the expression of the CCL5-CCR5 axis in the peripheral blood and oral tissue of healthy controls and patients with OLP using quantitative real-time PCR, Simple Western assays, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. In addition, we investigated the effects of the CCL5-CCR5 axis on the proliferation, apoptosis and migration of OLP T cells using Cell Counting Kit-8 (CCK8) assay, flow cytometry and transwell assay. We found that the expression of the CCL5-CCR5 axis was significantly elevated both in the peripheral blood and oral tissue of patients with OLP compared with healthy controls. CCL5 not only promoted OLP T-cell proliferation and migration but also inhibited OLP T-cell apoptosis. Moreover, CCR5 inhibition suppressed OLP T-cell proliferation and migration, whereas OLP T-cell apoptosis was promoted. In conclusion, our data suggest that the CCL5-CCR5 axis may be closely related to the inflammatory infiltration of T cells in OLP.

• Authors: Jing Shan, Shan Li, Chen Wang, Lin Liu, Xuewei Wang, Dandan Zhu, Yuan Fan, Juanyong Xu
• Journal: Experimental dermatology (Exp Dermatol) Vol. 28 Issue 7 Pg. 816-821 (Jul 2019) ISSN: 1600-0625 [Electronic] Denmark
• PMID: 31006151 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• CCL5 protein, human
• CCR5 protein, human
• Chemokine CCL5
• Receptors, CCR5
• Receptors, Chemokine

Topics

• Adult
• Aged
• Apoptosis
• Cell Movement
• Cell Proliferation
• Chemokine CCL5 (blood, metabolism)
• Female
• Humans
• Inflammation
• Lichen Planus, Oral (blood, metabolism)
• Lymphocyte Activation
• Male
• Middle Aged
• Receptors, CCR5 (blood, metabolism)
• Receptors, Chemokine (metabolism)
• T-Lymphocytes (immunology)