Abstract | BACKGROUND: Improving the water solubility of hydrophobic photosensitizer and increasing its accumulation in tumor tissue are essential for in vivo photodynamic therapy ( PDT). Considering commercialization or clinical application in future, it will be promising to achieve these purposes by developing new agents with simple and non-toxic structure. RESULTS: We conjugated multiple chlorin e6 (Ce6) molecules to gelatin polymer, synthesizing two types of gelatin-Ce6 conjugates with different amounts of Ce6: gelatin-Ce6-2 and gelatin-Ce6-8. The resulting conjugates remained soluble in aqueous solutions for a longer time than hydrophobic Ce6. The conjugates could generate singlet oxygen and kill tumor cells upon laser irradiation. After intravenous injection into SCC-7 tumor-bearing mice, gelatin-Ce6-2 showed prolonged blood circulation and highly increased accumulation in tumor tissue as observed in real-time imaging in vivo. After laser irradiation, gelatin-Ce6-2 suppressed tumor growth completely and enabled improved PDT compared to free Ce6 and gelatin-Ce6-8. CONCLUSIONS: This work demonstrates that a simple structure based on photosensitizer and gelatin can highly improve water solubility and stability. Superior tumor tissue accumulation and increased therapeutic efficacy of gelatin-Ce6 during in vivo PDT showed its high potential for clinical application.
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Authors | Jihwan Son, Gawon Yi, Moon-Hwa Kwak, Seung Mok Yang, Jae Myung Park, Bo-In Lee, Myung-Gyu Choi, Heebeom Koo |
Journal | Journal of nanobiotechnology
(J Nanobiotechnology)
Vol. 17
Issue 1
Pg. 50
(Apr 05 2019)
ISSN: 1477-3155 [Electronic] England |
PMID | 30953510
(Publication Type: Journal Article)
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Chemical References |
- Chlorophyllides
- Drug Carriers
- Photosensitizing Agents
- Polymers
- Porphyrins
- chlorin-2
- Singlet Oxygen
- phytochlorin
- Gelatin
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Topics |
- Animals
- Cell Line, Tumor
- Cell Survival
- Chlorophyllides
- Drug Carriers
- Gelatin
(chemistry)
- Humans
- Mice
- Neoplasm Transplantation
- Photosensitizing Agents
(pharmacology)
- Phototherapy
- Polymers
(chemistry)
- Porphyrins
(chemistry, pharmacology)
- Singlet Oxygen
(metabolism)
- Solubility
- Tissue Distribution
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