Abstract |
Colonization by Staphylococcus aureus (S. aureus) has been implicated in many infectious and wound healing disorders. This study was performed to characterize the pathogenic role of S. aureus alpha- hemolysin (alpha-toxin) in corneal epithelial wound healing and infectious keratitis in the setting of a corneal wound. The effect of wild-type and isogenic Hla mutant (α- hemolysin gene deleted) S. aureus bacteria and conditioned media on corneal epithelial wound healing was tested in vitro using a scratch assay and in vivo using a murine epithelial debridement model. The invasiveness of wild-type and Hla mutant S. aureus was evaluated in vitro in human corneal epithelial cells and in vivo in a murine model of infectious keratitis following total epithelial debridement. S. aureus and its conditioned media significantly delayed epithelial wound closure both in vitro (P < 0.05) and in vivo (P < 0.05). The effect of S. aureus on wound healing was significantly diminished with the Hla mutant strain (P < 0.05). Likewise, compared to the wild-type strain, the Hla mutant strain demonstrated significantly reduced ability to invade corneal epithelial cells in vitro (P < 0.05) and infect murine corneas following total epithelial debridement in vivo (P < 0.05). In conclusion, S. aureus alpha- hemolysin plays a major role in the pathologic modulation of corneal epithelial wound healing and the intracellular invasion of the bacteria. Limiting colonization by S. aureus and/or blocking alpha- hemolysin may provide a therapeutic approach for corneal wound healing and infectious disorders.
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Authors | Ilham Putra, Behnam Rabiee, Khandaker N Anwar, Sanaz Gidfar, Xiang Shen, Mehrdad Babalooee, Mahmood Ghassemi, Neda Afsharkhamseh, Saaquib Bakhsh, Dominique Missiakas, Ali Nezamabadi, Behrad Milani, Medi Eslani, Ali R Djalilian |
Journal | Experimental eye research
(Exp Eye Res)
Vol. 181
Pg. 263-270
(04 2019)
ISSN: 1096-0007 [Electronic] England |
PMID | 30822400
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2019 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Animals
- Corneal Diseases
(microbiology, pathology)
- Disease Models, Animal
- Epithelial Cells
(microbiology)
- Epithelium, Corneal
(injuries, microbiology)
- Hemolysin Proteins
(physiology)
- Humans
- Keratitis
(microbiology)
- Mice
- Mice, Inbred C57BL
- Staphylococcal Infections
(microbiology, pathology)
- Staphylococcus aureus
(pathogenicity)
- Wound Healing
(physiology)
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