The Effect of Chyle Fat Injection on Human Hypertrophic Scars in an Animal Model: A New Strategy for the Treatment of Hypertrophic Scars.

Abstract

BACKGROUND:

Chyle fat transplantation has shown positive effects on preexisting human hypertrophic scars (HSs) in a nude mouse HS graft model.

METHODS:

Hypertrophic scar fragments were obtained from 5 surgically treated burn patients and implanted into the backs of nude mice in 3 groups: group A, control; group B, triamcinolone; and group C, chyle fat. The specimens were implanted after the corresponding intralesional injection in each group, and the mice were observed for 4 weeks. In total, 18 mice and 72 scar specimens were studied. After 4 weeks, the HSs were removed from the mice. Then, the scar weights, histology, and decorin staining were assessed to evaluate the therapeutic efficacy.

RESULTS:

An obviously significant difference was observed in the HS weight reduction between groups A and C (P < 0.01), and a significant difference in the HS weight reduction was observed between groups A and B (P < 0.05). However, there was no significant difference between groups B and C. The treatment groups (groups B and C) showed strong decorin staining. Furthermore, the decorin staining was much stronger in group C than in group B (P < 0.05). Significant differences in extracellular matrix deposition were observed among the 3 groups, as determined by Masson trichrome staining. Both groups B and C showed significant therapeutic efficacy compared with group A, and group C exhibited a significant therapeutic effect compared with group B (P < 0.05).

CONCLUSIONS:

This study indicates that chyle fat grafting is beneficial for treating HSs.

• Authors: Junnan Chen, Linying Lai, Kui Ma, Xiao Xu, Zhenya Huang, Guiwen Zhou, Yunchao Zhou, Liming Liang, Minliang Chen
• Journal: Annals of plastic surgery (Ann Plast Surg) Vol. 82 Issue 6 Pg. 622-627 (06 2019) ISSN: 1536-3708 [Electronic] United States
• PMID: 30633019 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Triamcinolone

Topics

• Adipocytes (transplantation)
• Adipose Tissue (transplantation)
• Animals
• Burns (complications, therapy)
• Chyle
• Cicatrix, Hypertrophic (pathology, therapy)
• Disease Models, Animal
• Follow-Up Studies
• Humans
• Injections, Intralesional
• Male
• Mice
• Mice, Nude
• Random Allocation
• Risk Assessment
• Time Factors
• Tissue and Organ Harvesting (methods)
• Treatment Outcome
• Triamcinolone (therapeutic use)
• Wound Healing (physiology)