Distribution kinetics of puerarin in rat hippocampus after acute local cerebral ischemia.

Abstract

Puerarin, isolated from the roots of Pueraria lobata, is widely used for treating cerebral ischemia in China. The time- and dose-dependent distribution characteristics of puerarin in the ischemic hippocampus are unknown. In this study, puerarin concentration was determined by an indirect competitive ELISA using anti-puerarin monoclonal antibody. Area under the curve (AUC0-120 min) of puerarin (80 mg/kg) in the embolic hippocampus was higher than that in the normal hippocampus; the increase was significant only at 40 and 20 mg/kg. The maximum concentration (Cmax) of puerarin in the embolic hippocampus was higher than that in the normal hippocampus at all doses. The increase in both AUC0-120 min and Cmax was dose-dependent. Time to reach the maximum concentration (Tmax) of puerarin in the embolic and normal hippocampus was similar. Although the mean residence time in the embolic hippocampus differed from that in the normal hippocampus at 40 and 80 mg/kg, it was higher in the embolic hippocampus than in the normal hippocampus at 20 mg/kg. This is the first study to report that the time- and dose-dependent distribution characteristics of puerarin in the normal and embolic hippocampus after middle cerebral artery occlusion in rats dictate puerarin dose and duration to treat stroke.

• Authors: Hui Kong, Guiliang Zhang, Jinjun Cheng, Rongfeng Shi, Meiling Zhang, Peng Cao, Yan Zhao, Huihua Qu, Qingguo Wang
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 164 Pg. 196-201 (Feb 05 2019) ISSN: 1873-264X [Electronic] England
• PMID: 30390562 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier B.V.

Chemical References

• Isoflavones
• Vasodilator Agents
• puerarin

Topics

• Acute Disease (therapy)
• Animals
• Area Under Curve
• Brain Ischemia (drug therapy, etiology, pathology)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay (methods)
• Hippocampus (blood supply, chemistry, pathology)
• Infarction, Middle Cerebral Artery (complications)
• Injections, Intraperitoneal
• Isoflavones (administration & dosage, pharmacokinetics)
• Plant Roots (chemistry)
• Pueraria (chemistry)
• Rats
• Time Factors
• Tissue Distribution
• Vasodilator Agents (administration & dosage, pharmacokinetics)