Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA).

Abstract

BACKGROUND:

Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appear as promising tool.

OBJECTIVES:

This study evaluated the antimicrobial activity of different AMPs (Citropin 1.1, Temporin A, Pexiganan, CA(1-7)M(2-9)NH2, Pal-KGK-NH2, Pal-KKKK-NH2, LL-37) against human MRSA clinical isolates.

METHODS:

The Minimum Inhibitory Concentration (MIC) was assessed for each AMP; then, the most active ones (Citropin 1.1, Temporin A, CA(1-7)M(2-9)NH2 and Pal-KGK-NH2) were tested against selected MRSA strains by time-kill studies.

RESULTS:

The lowest MIC value was observed for Pal-KGK-NH2 (1 µg/ml), followed by Temporin A (4- 16 µg/ml), CA(1-7)M(2-9)NH2 (8-16 µg/ml) and Citropin 1.1 (16-64 µg/ml), while higher MICs were evidenced for LL-37, Pexiganan and Pal-KKKK-NH2 (> 128 µg/ml). In time-kill experiments, Citropin 1.1 and CA(1-7)M(2-9)NH2 showed a relatively high percentage of growth inhibition (>30 %) for all the tested MRSA clinical isolates, with a dose-dependent activity resulting in the highest percentage of bacterial growth inhibition (89.39%) at 2MIC concentration.

CONCLUSION:

Overall, our data demonstrated the potential of some AMPs against MRSA isolates, such as Citropin 1.1 and CA(1-7)M(2-9)NH2, that represents a promising area of development for different clinical applications.

• Authors: Eleonora Ciandrini, Gianluca Morroni, Daniela Arzeni, Wojciech Kamysz, Damian Neubauer, Elzbieta Kamysz, Oscar Cirioni, Lucia Brescini, Wally Baffone, Raffaella Campana
• Journal: Current topics in medicinal chemistry (Curr Top Med Chem) Vol. 18 Issue 24 Pg. 2116-2126 ( 2018) ISSN: 1873-4294 [Electronic] United Arab Emirates
• PMID: 30345920 (Publication Type: Journal Article)
• Copyright: Copyright© Bentham Science Publishers; For any queries, please email at [email protected].

Chemical References

• Anti-Bacterial Agents
• Antimicrobial Cationic Peptides

Topics

• Anti-Bacterial Agents (chemistry, pharmacology)
• Antimicrobial Cationic Peptides (chemistry, pharmacology)
• Dose-Response Relationship, Drug
• Methicillin-Resistant Staphylococcus aureus (drug effects)
• Microbial Sensitivity Tests
• Structure-Activity Relationship