Hsa-miR-203 inhibits fracture healing via targeting PBOV1.

Abstract

OBJECTIVE:

To explore the role of hsa-miR-203 in fracture healing and its underlying mechanism.

PATIENTS AND METHODS:

Expression levels of hsa-miR-203 and PBOV1 in patients with hand fractures and intra-articular fractures after treatment were detected by quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR). Viability and apoptosis of osteoblast cell line hFOB1.19 after hsa-miR-203 overexpression or knockdown were detected by cell counting kit-8 (CCK-8) assay and flow cytometry, respectively. The target gene of hsa-miR-203 was predicted by bioinformatics and verified by dual-luciferase reporter gene assay. Rescue experiments were conducted to further verify whether hsa-miR-203 could participate in fracture healing via PBOV1.

RESULTS:

No significant hsa-miR-203 expression was found in patients with hand fractures and intra-articular fractures after treatment for 7 days, which was remarkably upregulated on the 14th day. PBOV1 expression was gradually downregulated as treatment time prolongation. Overexpression of hsa-miR-203 decreased cell viability, but induced apoptosis of hFOB1.19 cells. Bioinformatics predicted that PBOV1 might be the target gene of hsa-miR-203, which was further verified by dual-luciferase reporter gene assay. The effect of hsa-miR-203 on viability and apoptosis of hFOB1.19 cells was reversed after the PBOV1 knockdown.

CONCLUSIONS:

Hsa-miR-203 inhibits fracture healing by regulating osteoblast viability and apoptosis via targeting PBOV1.

• Authors: S-Y Zhang, F Gao, C-G Peng, C-J Zheng, M-F Wu
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 22 Issue 18 Pg. 5797-5803 (09 2018) ISSN: 2284-0729 [Electronic] Italy
• PMID: 30280758 (Publication Type: Journal Article)

Chemical References

• MIRN203 microRNA, human
• MicroRNAs
• Neoplasm Proteins
• PBOV1 protein, human

Topics

• Apoptosis (physiology)
• Cell Proliferation (physiology)
• Cell Survival (physiology)
• Cells, Cultured
• Down-Regulation
• Fracture Healing (physiology)
• Humans
• MicroRNAs (biosynthesis, blood, physiology)
• Neoplasm Proteins (biosynthesis, blood)
• Osteoblasts (metabolism, physiology)
• Time Factors
• Up-Regulation