Apoptosis and autophagy induced by DVDMs-PDT on human esophageal cancer Eca-109 cells.

Abstract

BACKGROUND:

Esophageal cancer is a common gastrointestinal cancer. About 300,000 people die from esophageal cancer every year in the world. Photodynamic therapy (PDT) has attracted attention as a feasible cancer therap for this diagnosis. Sinoporphyrin sodium (DVDMs) is a novel sensitizer isolated from photofrin. In this study, we aimed to investigate the effects of DVDMs mediated photodynamic therapy and the possible mechanism on human esophageal cancer Eca-109 cells.

METHODS:

Cell viability was measured by MTT assay and cell apoptosis was determined by Annexin V-PE/7-AAD and western blot. MDC staining and western blot were used to evaluate cell autophagy. The production of intracellular reactive oxygen species (ROS) was detected by flow cytometry. The expression of MAPK and HO-1 were detected by western blot.

RESULTS:

DVDMs-PDT decreased cell viability and induced cell apoptosis and autophagy. Autophagy inhibition reduced cell apoptosis triggered by DVDMs-PDT in Eca-109 cells. Generation of ROS was detected in DVDMs-PDT group. p38MAPK, JNK and HO-1 were activated after PDT treatment and the activation were reversed by adding ROS scavenger NAC.

CONCLUSIONS:

Our studies demonstrated that DVDMs-PDT induced apoptosis and autophagy in Eca-109 cells. DVDMs-PDT induced ROS generation in Eca-109 cells, and the generation of ROS activated p38MAPK and JNK. Activation of p38MAPK and JNK may be involved in PDT-induced apoptosis.

• Authors: Yin Shi, Boli Zhang, Xiaolan Feng, Fei Qu, Shuang Wang, Lijie Wu, Xiaobing Wang, Quanhong Liu, Pan Wang, Kun Zhang
• Journal: Photodiagnosis and photodynamic therapy (Photodiagnosis Photodyn Ther) Vol. 24 Pg. 198-205 (Dec 2018) ISSN: 1873-1597 [Electronic] Netherlands
• PMID: 30268863 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Chemical References

• Photosensitizing Agents
• Porphyrins
• Reactive Oxygen Species
• sinoporphyrin sodium
• HMOX1 protein, human
• Heme Oxygenase-1
• p38 Mitogen-Activated Protein Kinases

Topics

• Apoptosis (drug effects)
• Autophagy (drug effects)
• Cell Line, Tumor
• Esophageal Neoplasms (drug therapy)
• Heme Oxygenase-1 (biosynthesis)
• Humans
• Lasers, Semiconductor
• Photochemotherapy (methods)
• Photosensitizing Agents (pharmacology)
• Porphyrins (pharmacology)
• Reactive Oxygen Species
• p38 Mitogen-Activated Protein Kinases (biosynthesis)