Genome-Wide Chromatin Structure Changes During Adipogenesis and Myogenesis.

Abstract

The recently developed high-throughput chromatin conformation capture (Hi-C) technology enables us to explore the spatial architecture of genomes, which is increasingly considered an important regulator of gene expression. To investigate the changes in three-dimensional (3D) chromatin structure and its mediated gene expression during adipogenesis and myogenesis, we comprehensively mapped 3D chromatin organization for four cell types (3T3-L1 pre-adipocytes, 3T3-L1-D adipocytes, C2C12 myoblasts, and C2C12-D myotubes). We demonstrate that the dynamic spatial genome architecture affected gene expression during cell differentiation. A considerable proportion (~22%) of the mouse genome underwent compartment A/B rearrangement during adipogenic and myogenic differentiation, and most (~80%) upregulated marker genes exhibited an active chromatin state with B to A switch or stable A compartment. More than half (65.4%-73.2%) of the topologically associating domains (TADs) are dynamic. The newly formed TAD and intensified local interactions in the Fabp gene cluster indicated more precise structural regulation of the expression of pro-differentiation genes during adipogenesis. About half (32.39%-59.04%) of the differential chromatin interactions (DCIs) during differentiation are promoter interactions, although these DCIs only account for a small proportion of genome-wide interactions (~9.67% in adipogenesis and ~4.24% in myogenesis). These differential promoter interactions were enriched with promoter-enhancer interactions (PEIs), which were mediated by typical adipogenic and myogenic transcription factors. Differential promoter interactions also included more differentially expressed genes than nonpromoter interactions. Our results provide a global view of dynamic chromatin interactions during adipogenesis and myogenesis and are a resource for studying long-range chromatin interactions mediating the expression of pro-differentiation genes.

• Authors: Mengnan He, Yan Li, Qianzi Tang, Diyan Li, Long Jin, Shilin Tian, Tiandong Che, Shen He, Lamei Deng, Guangliang Gao, Yiren Gu, Zhi Jiang, Xuewei Li, Mingzhou Li
• Journal: International journal of biological sciences (Int J Biol Sci) Vol. 14 Issue 11 Pg. 1571-1585 ( 2018) ISSN: 1449-2288 [Electronic] Australia
• PMID: 30263009 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chromatin

Topics

• 3T3-L1 Cells
• Adipogenesis (genetics, physiology)
• Animals
• Cell Line
• Cell Nucleus (metabolism)
• Chromatin (metabolism)
• Chromatin Assembly and Disassembly (genetics)
• Genome (genetics)
• Mice
• Muscle Development (genetics, physiology)