Glycomimetic-Conjugated Photosensitizer for Specific Pseudomonas aeruginosa Recognition and Targeted Photodynamic Therapy.

Abstract

Due to the rapid development of bacterial resistance, there is an urgent need to explore new antibacterial agents to substitute for traditional antibiotic therapy. Photodynamic therapy has been identified as a promising bactericidal method to conquer antibiotic-resistant pathogens. To solve the problem of photosensitizer damage to normal tissues in vivo, we developed a boron-dipyrrolemethene (BODIPY)-based glycosylated photosensitizer for ablating Pseudomonas aeruginosa ( P. aeruginosa). This glycosylated photosensitizer exhibited good water solubility and generated 1O2 rapidly in an aqueous solution under light exposure. The photosensitizer did not cause detectable toxicity to human cells in the dark. Importantly, the photosensitizer was able to selectively attach to P. aeruginosa over normal cells, thus resulting in effective pathogen ablation by reactive oxygen species. Moreover, the photosensitizer inhibited over 90% of the biofilm formation produced by P. aeruginosa. The results indicate that the design of the macromolecular photosensitizer-induced bacterial death and inhibited biofilm formation provide a novel strategy for overcoming bacterial infection.

• Authors: Yu Zhao, Zhentan Lu, Xiaomei Dai, Xiaosong Wei, Yunjian Yu, Xuelei Chen, Xinge Zhang, Chaoxing Li
• Journal: Bioconjugate chemistry (Bioconjug Chem) Vol. 29 Issue 9 Pg. 3222-3230 (09 19 2018) ISSN: 1520-4812 [Electronic] United States
• PMID: 30152991 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Photosensitizing Agents
• Water
• Galactose

Topics

• Anti-Bacterial Agents (chemistry, pharmacology)
• Biofilms (drug effects)
• Drug Resistance, Bacterial
• Galactose (chemistry)
• Glycosylation
• Microscopy, Electron, Scanning
• Microscopy, Electron, Transmission
• Photochemotherapy
• Photosensitizing Agents (chemical synthesis, chemistry, pharmacology, toxicity)
• Pseudomonas aeruginosa (drug effects, physiology, ultrastructure)
• Solubility
• Water