Enhancement of O2- generation and tumoricidal activity of murine macrophages by a monosaccharide precursor of Escherichia coli lipid A.

Abstract

The effects of a monosaccharide precursor of Escherichia coli lipid A (lipid X) on murine macrophages were studied. Lipid X is a diacylglucosamine 1-phosphate bearing beta-hydroxymyristoyl groups at positions 2 and 3. Lipid X, as well as lipopolysaccharide and lipid A, enhanced O2- generation in mouse peritoneal macrophages and a macrophage-like cell line, J774.1, and further induced the tumor-cytotoxic activity of peritoneal macrophages. Elimination of a 1-phosphate or 3-O-beta-hydroxymyristoyl groups are essential for the elevated O2- generation and induction of tumoricidal activity due to lipid X.

• Authors: F Amano, M Nishijima, K Akagawa, Y Akamatsu
• Journal: FEBS letters (FEBS Lett) Vol. 192 Issue 2 Pg. 263-6 (Nov 18 1985) ISSN: 0014-5793 [Print] England
• PMID: 2998869 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycolipids
• Lipid A
• Lipopolysaccharides
• Superoxides
• lipid X

Topics

• Animals
• Cell Line
• Cytotoxicity, Immunologic (drug effects)
• Escherichia coli (immunology)
• Glycolipids (pharmacology)
• Leukemia, Experimental (immunology)
• Lipid A (pharmacology)
• Lipopolysaccharides (pharmacology)
• Macrophage Activation (drug effects)
• Macrophages (drug effects, immunology, metabolism)
• Mice
• Mice, Inbred ICR
• Superoxides (metabolism)