De Novo Transcriptome Sequencing of the Deep-Sea-Derived Fungus Dichotomomyces cejpii and Analysis of Gliotoxin Biosynthesis Genes.

Abstract

Gliotoxin, produced by fungi, is an epipolythiodioxopiperazine (ETP) toxin with bioactivities such as anti-liver fibrosis, antitumor, antifungus, antivirus, antioxidation, and immunoregulation. Recently, cytotoxic gliotoxins were isolated from a deep-sea-derived fungus, Dichotomomyces cejpii. However, the biosynthetic pathway for gliotoxins in D. cejpii remains unclear. In this study, the transcriptome of D. cejpii was sequenced using an Illumina Hiseq 2000. A total of 19,125 unigenes for D. cejpii were obtained from 9.73 GB of clean reads. Ten genes related to gliotoxin biosynthesis were annotated. The expression levels of gliotoxin-related genes were detected through quantitative real-time polymerase chain reaction (qRT-PCR). The GliG gene, encoding a glutathione S-transferase (DC-GST); GliI, encoding an aminotransferase (DC-AI); and GliO, encoding an aldehyde reductase (DC-AR), were cloned and expressed, purified, and characterized. The results suggested the important roles of DC-GST, DC-AT, and DC-AR in the biosynthesis of gliotoxins. Our study on the genes related to gliotoxin biosynthesis establishes a molecular foundation for the wider application of gliotoxins from D. cejpii in the biomedical industry in the future.

• Authors: Wei Ye, Weimin Zhang, Taomei Liu, Zilei Huang, Muzi Zhu, Yuchan Chen, Haohua Li, Saini Li
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 19 Issue 7 (Jun 29 2018) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 29966253 (Publication Type: Journal Article)

Chemical References

• Gliotoxin
• Aldehyde Reductase
• Glutathione Transferase

Topics

• Aldehyde Reductase (genetics)
• Fungi (genetics, metabolism)
• Gene Expression Profiling (methods)
• Gliotoxin (biosynthesis)
• Glutathione Transferase (genetics)
• Transcriptome (genetics)