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Elevated Krüppel-like factor 5 expression in spatiotemporal mouse lungs is similar to human congenital cystic adenomatoid malformation of the lungs.

Abstract
Objective The study aimed to investigate the role of high Krüppel-like factor 5 (KLF5) expression on the pathogenesis of congenital cystic adenomatoid malformation of the lungs (CCAML) in mice. Methods A mouse model of high KLF5 expression in the lungs was established. KLF5 expression and the pulmonary lumen diameter were examined by immunohistochemistry to determine a successful model. Basement membrane damage and activity of matrix metalloproteinase-9 (MMP-9) were examined. After an adenovirus carrying KLF5 gene transfection in lung adenocarcinoma (H441) was created, changes in expression and activity of MMP-9 were determined. Results In a mouse model with high KLF5 expression, the pulmonary lumen was markedly enlarged, indicating establishment of CCAML. The basement membrane was degraded, and MMP-9 activity was significantly higher in the model group compared with the control group. Moreover, mice in a cellular model after transfection also showed higher MMP-9 activity than did controls. Conclusion High KLF5 expression may play a pivotal role in the pathogenesis of CCAML, partly through regulating the activity of MMP-9.
AuthorsXueyan Wang, Huajing Wan, Shuo Yang, Rong Zhou, Yong Liao, Fan Wang, Ximin Chen, Zhiling Wu
JournalThe Journal of international medical research (J Int Med Res) Vol. 46 Issue 7 Pg. 2856-2865 (Jul 2018) ISSN: 1473-2300 [Electronic] England
PMID29896983 (Publication Type: Journal Article)
Chemical References
  • Klf5 protein, mouse
  • Kruppel-Like Transcription Factors
  • Matrix Metalloproteinase 9
Topics
  • Animals
  • Cesarean Section
  • Cystic Adenomatoid Malformation of Lung, Congenital (genetics, metabolism)
  • Disease Models, Animal
  • Female
  • Gene Expression
  • Humans
  • Immunohistochemistry
  • Kruppel-Like Transcription Factors (biosynthesis, genetics)
  • Lung (metabolism)
  • Matrix Metalloproteinase 9 (metabolism)
  • Mice
  • Pregnancy

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