Abstract |
In this paper, the design, synthesis, and molecular modeling of a new azole-based HO-1 inhibitors was reported, using compound 1 as a lead compound, in which an imidazole moiety is linked to a hydrophobic group by means of an ethanolic spacer. The tested compounds showed a good inhibitor activity and possessed IC50 values in the micromolar range. These results were obtained by targeting the hydrophobic western region. Molecular modeling studies confirmed a consolidated binding mode in which the nitrogen of the imidazolyl moiety coordinated the heme ferrous iron, meanwhile the hydrophobic groups were located in the western region of HO-1 binding pocket. Moreover, the new compounds were screened for in silico ADME-Tox properties to predict drug-like behavior with convincing results. Finally, the in vitro antitumor activity profile of compound 1 was investigated in different cancer cell lines and nanomicellar formulation was synthesized with the aim of improving compound's 1 water solubility. Finally, compound 1 was tested in melanoma cells in combination with doxorubicin showing interesting synergic activity.
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Authors | Khaled F Greish, Loredana Salerno, Reem Al Zahrani, Emanuele Amata, Maria N Modica, Giuseppe Romeo, Agostino Marrazzo, Orazio Prezzavento, Valeria Sorrenti, Antonio Rescifina, Giuseppe Floresta, Sebastiano Intagliata, Valeria Pittalà |
Journal | Molecules (Basel, Switzerland)
(Molecules)
Vol. 23
Issue 5
(May 18 2018)
ISSN: 1420-3049 [Electronic] Switzerland |
PMID | 29783634
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Imidazoles
- Micelles
- Heme Oxygenase-1
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Topics |
- Antineoplastic Agents
(chemistry, pharmacology)
- Binding Sites
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Drug Screening Assays, Antitumor
- Heme Oxygenase-1
(antagonists & inhibitors)
- Humans
- Hydrophobic and Hydrophilic Interactions
- Imidazoles
(chemistry, pharmacology)
- Micelles
- Molecular Docking Simulation
- Particle Size
- Structure-Activity Relationship
- Surface Properties
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