Abstract |
Tumor growth is fueled by subset of cells with stem cell properties (Cancer stem cells, CSCs). While persistent activation of Wnt/β- catenin signaling confers CSC properties, it remains unclear how epigenetic modifications regulate Wnt target genes to dictate their self-renewal. Here, we report a novel Wnt-responsive epigenetic switch for CSC maintenance through activating the stem cell transcription factor ASCL2 in gastric carcinoma (GC). We characterize ASCL2-expressing (ASCL2+) GC cells as a subset of Wnt-responsive CSCs that depend on ASCL2 for self-renewal. High-throughput RNAi screening uncovers that the histone methyltransferase SMYD3 determines H3K4me3 status at the ASCL2 locus to promote ASCL2 expression. Moreover, SMYD3 may be transcriptionally activated by the β- catenin/TCF4 complex, indicating that the SMYD3-ASCL2 axis may be an integral component of Wnt signaling. Consistently, SMYD3 maintains self-renewal and tumorigenicity of ASCL2+ CSCs largely through inducing ASCL2. Clinically, overexpression of SMYD3 and ASCL2 are associated with malignant progression and poor patient outcomes in GC. Together, these findings define a Wnt-responsive CSC pathway that could be exploited to identify essential regulators of the signaling output, and reveal SMYD3 as an epigenetic target for eliminating CSCs in human cancers.
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Authors | Tao Wang, Hong Wu, Sha Liu, Zengjie Lei, Zhongyi Qin, Liangzhi Wen, Kaijun Liu, Xingwei Wang, Yan Guo, Qin Liu, Lei Liu, Jun Wang, Li Lin, Chengyi Mao, Xiangfeng Zhu, Hualiang Xiao, Xiuwu Bian, Dongfeng Chen, Chuan Xu, Bin Wang |
Journal | Cancer letters
(Cancer Lett)
Vol. 430
Pg. 11-24
(08 28 2018)
ISSN: 1872-7980 [Electronic] Ireland |
PMID | 29746925
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2018 Elsevier B.V. All rights reserved. |
Chemical References |
- ASCL2 protein, human
- Basic Helix-Loop-Helix Transcription Factors
- CTNNB1 protein, human
- Histones
- RNA, Small Interfering
- Wnt Proteins
- beta Catenin
- histone H3 trimethyl Lys4
- Histone-Lysine N-Methyltransferase
- SMYD3 protein, human
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Topics |
- Basic Helix-Loop-Helix Transcription Factors
(genetics, metabolism)
- Carcinogenesis
(genetics)
- Carcinoma
(genetics, mortality, pathology)
- Cell Line, Tumor
- Cell Self Renewal
(genetics)
- Epigenesis, Genetic
- Female
- Gene Expression Regulation, Neoplastic
- Gene Knockdown Techniques
- Histone-Lysine N-Methyltransferase
(genetics, metabolism)
- Histones
(metabolism)
- Humans
- Male
- Middle Aged
- Neoplastic Stem Cells
(metabolism)
- RNA, Small Interfering
(metabolism)
- Stomach
(pathology)
- Stomach Neoplasms
(genetics, mortality, pathology)
- Survival Analysis
- Wnt Proteins
(metabolism)
- Wnt Signaling Pathway
(genetics)
- beta Catenin
(genetics, metabolism)
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