[Growth Regulation of Factor Inhibiting Hypoxia-Inducible Factor in Renal Carcinoma Cells].

Abstract

OBJECTIVE:

To detect the expression and growth regulation effects of the factor inhibiting hypoxia-inducible factor (FIH-1) in renal carcinoma cells.

METHODS:

The expressions of FIH-1,hypoxia inducible factor (HIF) and vascular endothelial growth factor (VEGF) were examined in OS-RC-2 and 786-0 cell lines via real-time PCR (RT-PCR) and Western blot. Overexpression plasmid of FIH-1 were designed and constructed as EX-V1104-M45-FIH-1. After the transfection of the plasmid into OS-RC-2 and 786-0 cells,the expression levels of FIH-1,HIF and VEGF were measured by RT-PCR and Western blot,while cell proliferation was tested by MTT.

RESULTS:

The expressions of FIH-1,HIF-1α,HIF-2α and VEGF were detected in OS-RC-2 cells,and FIH-1,HIF-2α and VEGF were detected in 786-0 cells. After the transfection of EX-V1104-M45-FIH-1, the level of FIH-1 was increased in OS-RC-2,while the levels of HIF-2α,HIF-1α and VEGF were decreased. MTT showed that FIH-1 plasmid transfection decreased the cell proliferation of OS-RC-2,but did not show inhibition on 786-0 cells proliferation.

CONCLUSION:

FIH-1/HIF-1α/VEGF signaling pathway exists in renal carcinoma cell lines.

• Authors: Shi Deng, Bo Yang, Zheng-Ju Ren, Qiang Dong
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 49 Issue 1 Pg. 29-33 (Jan 2018) ISSN: 1672-173X [Print] China
• PMID: 29737085 (Publication Type: Journal Article)
• Copyright: CopyrightCopyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).

Chemical References

• Basic Helix-Loop-Helix Transcription Factors
• HIF1A protein, human
• Hypoxia-Inducible Factor 1, alpha Subunit
• Repressor Proteins
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• endothelial PAS domain-containing protein 1
• Mixed Function Oxygenases
• HIF1AN protein, human

Topics

• Basic Helix-Loop-Helix Transcription Factors (metabolism)
• Carcinoma, Renal Cell (metabolism)
• Cell Line, Tumor
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Kidney Neoplasms (metabolism)
• Mixed Function Oxygenases (metabolism)
• Repressor Proteins (metabolism)
• Signal Transduction
• Transfection
• Vascular Endothelial Growth Factor A (metabolism)