Testing Cell-Based Immunotherapy for Colorectal Cancer.

Abstract

Cell-based immunotherapy for cancer is emerging as an attractive alternative to conventional small-molecule or antibody-based treatment. Due to the characteristics of cell-based therapy, validation of test materials before in vivo administration is required. Here we describe general validation steps for preclinical evaluation of cell-based immunotherapy. We also describe a xenograft model of human colorectal cancer. This model can be used for applied to preclinical evaluation of various cell-based therapy regimens for colorectal cancer treatment.

• Authors: Jong Soon Kang, Yeo Dae Yoon, Myeong Youl Lee, Chang Woo Lee, Suk Jong Lee, Sang-Bae Han
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1765 Pg. 299-305 ( 2018) ISSN: 1940-6029 [Electronic] United States
• PMID: 29589316 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Cell Culture Techniques (instrumentation, methods)
• Cell Line, Tumor
• Cell Survival
• Cell Transplantation (instrumentation, methods)
• Colorectal Neoplasms (immunology, therapy)
• Dendritic Cells (immunology, transplantation)
• Flow Cytometry (instrumentation, methods)
• Humans
• Immunotherapy (instrumentation, methods)
• Killer Cells, Natural (immunology, transplantation)
• Mice
• Mice, Nude
• Mice, SCID
• T-Lymphocytes (immunology, transplantation)
• Validation Studies as Topic
• Xenograft Model Antitumor Assays (instrumentation, methods)