lncRNA-NKILA/NF-κB feedback loop modulates laryngeal cancer cell proliferation, invasion, and radioresistance.

Abstract

Laryngeal cancer is one of the most common head and neck malignant tumors and is commonly resistant to X-ray-based radiotherapy. NF-κB interacting lncRNA (NKILA) has been reported to serve as a tumor suppressor in several cancers through combining with NF-κB: IκB complex thereby inhibiting NF-κB activation. Herein, we demonstrated a low NKILA expression in laryngeal cancer and its correlation with shorter overall survival in patients with laryngeal cancer. NKILA serves as a tumor suppressor in laryngeal cancer by suppressing laryngeal cancer cell viability and migration, whereas promoting cell apoptosis; NKILA knockdown reverses the cytotoxicity of X-ray radiation on laryngeal cancer cells through combining with NF-κB: IκB complex to inhibit IκB phosphorylation, inhibit p65 nuclear translocation, and finally inhibit NF-κB activation. NF-κB binds to the promoter region of NKILA to activate its transcriptional activity, upregulated NKILA then inhibits IκB phosphorylation and NF-κB activation, thus forming a negative feedback loop to sensitize laryngeal cancer cell to X-ray radiation. In conclusion, NKILA can serve as a promising agent of enhancing the cytotoxicity of X-ray radiation on laryngeal cancer and addressing the radioresistance of laryngeal cancer.

• Authors: Tao Yang, Shisheng Li, Jiajia Liu, Danhui Yin, Xinming Yang, Qinglai Tang
• Journal: Cancer medicine (Cancer Med) Vol. 7 Issue 5 Pg. 2048-2063 (05 2018) ISSN: 2045-7634 [Electronic] United States
• PMID: 29573243 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

Chemical References

• RELA protein, human
• RNA, Long Noncoding
• Transcription Factor RelA
• long noncoding RNA NKILA, human
• I-kappa B Kinase

Topics

• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Enzyme Activation (genetics)
• Female
• Gene Expression (genetics)
• Humans
• I-kappa B Kinase (metabolism)
• Laryngeal Neoplasms (genetics, mortality, radiotherapy)
• Male
• Middle Aged
• Phosphorylation (genetics)
• Promoter Regions, Genetic (genetics)
• RNA, Long Noncoding (genetics)
• Radiation Tolerance (genetics)
• Transcription Factor RelA (genetics)