Rebamipide suppresses TNF-α production and macrophage infiltration in the conjunctiva.

Abstract

PURPOSE:

To evaluate the anti-inflammatory effect of rebamipide during corneal epithelial wound healing using a mouse wound repair model.

METHODS:

A 2-mm circular disc of the central cornea was demarcated in the right eye of C57BL/6 mice and the epithelium removed. Rebamipide 2% eyedrop was instilled onto the wounded eye 5 times a day (n = 26). Phosphate-buffered saline (PBS) was used in the control group (n = 26). Corneal and conjunctival IL-1β and TNF-α levels were measured at 6 h and 24 h postinjury by ELISA. The wounded area was evaluated by fluorescein staining at 24 h postinjury. Macrophage infiltration was assessed immunohistochemically, and TNF-α secretion from macrophages was examined in vitro.

RESULTS:

Conjunctival IL-1β and corneal IL-1β levels were not significantly different between PBS-treated and rebamipide-treated groups. However, conjunctival TNF-α level was significantly lower in the rebamipide-treated group compared with the PBS-treated group. Macrophage migration into the conjunctiva, but not into the cornea, was suppressed by rebamipide treatment. In addition, TNF-α secretion from cultured macrophages was suppressed by rebamipide in a concentration-dependent manner. Rebamipide treatment significantly accelerated corneal epithelial wound healing at 24 h postinjury.

CONCLUSIONS:

In a mouse corneal epithelial wound model, rebamipide suppressed TNF-α secretion and macrophage infiltration in the conjunctiva, which might have contributed to accelerated corneal epithelial wound healing in the first 24 h following injury.

• Authors: Kazuki Tajima, Takaaki Hattori, Hiroki Takahashi, Haruki Katahira, Akitomo Narimatsu, Shigeto Kumakura, Hiroshi Goto
• Journal: Veterinary ophthalmology (Vet Ophthalmol) Vol. 21 Issue 4 Pg. 347-352 (Jul 2018) ISSN: 1463-5224 [Electronic] England
• PMID: 29251403 (Publication Type: Journal Article)
• Copyright: © 2017 American College of Veterinary Ophthalmologists.

Chemical References

• Anti-Inflammatory Agents
• Quinolones
• Tumor Necrosis Factor-alpha
• rebamipide
• Alanine

Topics

• Alanine (analogs & derivatives, pharmacology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Conjunctiva (chemistry, drug effects, metabolism)
• Cornea (chemistry, drug effects)
• Corneal Injuries (drug therapy)
• Enzyme-Linked Immunosorbent Assay
• Female
• Macrophages (drug effects)
• Mice
• Mice, Inbred C57BL
• Quinolones (pharmacology)
• Tumor Necrosis Factor-alpha (analysis, antagonists & inhibitors)
• Wound Healing (drug effects)