Inactivation of NSF ATPase Leads to Cathepsin B Release After Transient Cerebral Ischemia.

Abstract

Neurons have extraordinary large cell membrane surface area, thus requiring extremely high levels of intracellular membrane-trafficking activities. Consequently, defects in the membrane-trafficking activities preferentially affect neurons. A critical molecule for controlling the membrane-trafficking activities is the N-ethylmaleimide-sensitive factor (NSF) ATPase. This study is to investigate the cascade of events of NSF ATPase inactivation, resulting in a massive buildup of late endosomes (LEs) and fatal release of cathepsin B (CTSB) after transient cerebral ischemia using the 2-vessel occlusion with hypotension (2VO+Hypotension) global brain ischemia model. Rats were subjected to 20 min of transient cerebral ischemia followed by 0.5, 4, 24, and 72 h of reperfusion. Neuronal histopathology and ultrastructure were examined by the light and electron microscopy, respectively. Western blotting and confocal microscopy were utilized for analyzing the levels, redistribution, and co-localization of Golgi apparatus and endosome or lysosome markers. Transient cerebral ischemia leads to delayed neuronal death that occurs at 48-72 h of reperfusion mainly in hippocampal CA1 and neocortical (Cx) layers 3 and 5 pyramidal neurons. During the delayed period, NSF ATPase is irreversibly trapped into inactive protein aggregates selectively in post-ischemic neurons destined to die. NSF inactivation leads to a massive buildup of Golgi fragments, transport vesicles (TVs) and late endosomes (LEs), and release of the 33 kDa LE type of CTSB, which is followed by delayed neuronal death after transient cerebral ischemia. The results support a novel hypothesis that transient cerebral ischemia leads to NSF inactivation, resulting in a cascade of events of fatal release of CTSB and delayed neuronal death after transient cerebral ischemia.

• Authors: Dong Yuan, Chunli Liu, Jiang Wu, Bingren Hu
• Journal: Translational stroke research (Transl Stroke Res) Vol. 9 Issue 3 Pg. 201-213 (06 2018) ISSN: 1868-601X [Electronic] United States
• PMID: 29039034 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Membrane Transport Proteins
• Cathepsin B
• Ctsb protein, rat
• N-Ethylmaleimide-Sensitive Proteins

Topics

• Animals
• Cathepsin B (metabolism)
• Disease Models, Animal
• Endosomes (metabolism)
• Golgi Apparatus (metabolism)
• Intracellular Membranes (metabolism)
• Ischemic Attack, Transient (metabolism)
• Membrane Transport Proteins (metabolism)
• N-Ethylmaleimide-Sensitive Proteins (metabolism)
• Neurons (metabolism)
• Rats