Pharmaceutical Production of Anti-tumor and Immune-potentiating Enterococcus faecalis-2001 β-glucans: Enhanced Activity of Macrophage and Lymphocytes in Tumor-implanted Mice.

Abstract

BACKGROUND:

Enterococcus faecalis 2001 is a probiotic lactic acid bacterium and has been used as a biological response modifier (BRM). From physiological limitation of bacterial preservation in storage and safety, the live E. faecalis 2001 has been heat-treated and the BRM components containing high level of β-glucan, named EF-2001, were prepared.

METHOD:

The heat-treated EF-2001 has been examined for the antioxidative potential for radical scavenging and anti-tumor activities as well as immune-enhancing response in mice. Lymphocyte versus polymorphonuclear leukocyte ratio was increased in mice upon treatment with EF-2001. The number of lymphocytes was increased in the EF-2001-treated group. In the mice bearing two different Ehrlich solid and Sarcoma-180 carcinomas, the treatment with EF-2001 resulted in anti-tumor action. Tumor-suppressive capacity upon treatment with EF-2001 was significantly increased compared to normal controls.

RESULTS:

During the time interval administration of 5 weeks between the priming and secondary administration of EF-2001, the expression and production levels of TNF-α were also observed in the EF- 2001-administered mice. Additionally, anti-tumor activity examined with the intravenous administration of EF 2001 with a 34 times interval was also observed, as the growth of Sarcoma180 cells was clearly inhibited by the EF-2001.

CONCLUSION:

From the results, it was suggested that the immune response is enhanced due to antioxidative activity caused by the EF-2001 and anti-tumor activity by NK cells and TNF-α.

• Authors: Yeun-Hwa Gu, Hyunju Choi, Takenori Yamashita, Ki-Mun Kang, Masahiro Iwasa, Moon-Jo Lee, Kyoung Hae Lee, Cheorl-Ho Kim
• Journal: Current pharmaceutical biotechnology (Curr Pharm Biotechnol) Vol. 18 Issue 8 Pg. 653-661 (Nov 10 2017) ISSN: 1873-4316 [Electronic] Netherlands
• PMID: 28969561 (Publication Type: Journal Article)
• Copyright: Copyright© Bentham Science Publishers; For any queries, please email at [email protected].

Chemical References

• Antineoplastic Agents
• Antioxidants
• Immunologic Factors
• Tumor Necrosis Factor-alpha
• beta-Glucans

Topics

• Animals
• Antineoplastic Agents (isolation & purification, pharmacology)
• Antioxidants (isolation & purification, pharmacology)
• Carcinoma, Ehrlich Tumor (drug therapy, immunology)
• Enterococcus faecalis
• Immunologic Factors (isolation & purification, pharmacology)
• Killer Cells, Natural (drug effects)
• Lymphocytes (drug effects, immunology)
• Macrophages (drug effects, immunology)
• Male
• Mice, Inbred ICR
• Neutrophils (drug effects, immunology)
• Probiotics
• Sarcoma 180 (drug therapy, immunology)
• Tumor Necrosis Factor-alpha (analysis)
• beta-Glucans (isolation & purification, pharmacology)