Bioaugmentation of Zn solubilizing rhizobacteria could be a sustainable intervention to increase bioavailability of Zn in soil which can be helpful in mitigation of yield loss and
malnutrition of
zinc. In present study, a number of pure rhizobacterial colonies were isolated from maize rhizosphere and screened for their ability to solubilize
zinc oxide. These isolates were screened on the basis of
zinc and
phosphate solubilization, IAA production,
protease production,
catalase activity and
starch hydrolysis. All the selected isolates were also positive for
oxidase activity (except ZM22), HCN production (except ZM27) and utilization of
citrate. More than 70% of isolates produces
ammonia,
hydrogen cyanide,
siderophores, exopolysaccharides and
cellulase. More than half of isolates also showed potential for
urease activity and production of
lipase. The ZM31 and S10 were the only isolates which showed the
chitinase activity. All these isolates were evaluated in a jar trial for their ability to promote growth of maize under axenic conditions. Results revealed that inoculation of selected
zinc solubilizing rhizobacterial isolates improved the growth of maize. In comparison, isolates ZM20, ZM31, ZM63 and S10 were best compared to other tested isolates in stimulating the growth attributes of maize like shoot length, root length, plant fresh and dry biomass. These strains were identified as Bacillus sp. (ZM20), Bacillus aryabhattai (ZM31 and S10) and Bacillus subtilis (ZM63) through
16S rRNA sequencing. This study indicated that inoculation of Zn solubilizing strains have potential to promote growth and can be the potential bio-inoculants for biofortification of maize to overcome the problems of
malnutrition.