Can Ultrasound Imaging Predict the Success of an Experimental Steatofibrosis Model?

Abstract

Our goal was to evaluate the role of ultrasound (US) imaging in an experimental 2-hit steatofibrosis rat model. Nineteen female Sprague-Dawley rats were divided into 2 groups: control group (n = 6) and high-fat diet carbontetrachloride (HFD-CCl4) group (n = 13) that was fed with HFD for 14 weeks. Ultrasound was performed to evaluate liver steatosis. The HFD-CCl4 group rats were divided further into 2 subgroups: HFD rats with liver steatosis [US (+) group; n = 6] and without steatosis [US (-) group; n = 7]. All rats in the subgroups were administered with CCl4. In both US (+) and US (-) subgroups, steatosis score, fibrosis score, triglyceride, and hydroxyproline contents were markedly higher compared with the control group. When compared with the US (-) group, triglyceride and hydroxyproline contents were significantly higher in the US (+) group, whereas steatosis and fibrosis scores were not different. Ultrasound imaging may be useful to assess the success of a 2-hit experimental steatofibrosis model.

• Authors: Rustu Turkay, Abdurrahman Fatih Aydin, Ilknur Bingul, Canan Kucukgergin, Isin Dogan-Ekici, Elif Hocaoglu, Ercan Inci, Baris Bakir, Mujdat Uysal
• Journal: Ultrasound quarterly (Ultrasound Q) Vol. 33 Issue 2 Pg. 157-161 (Jun 2017) ISSN: 1536-0253 [Electronic] United States
• PMID: 28422772 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Carbon Tetrachloride

Topics

• Animals
• Carbon Tetrachloride
• Diet, High-Fat
• Disease Models, Animal
• Fatty Liver (diagnostic imaging)
• Female
• Humans
• Image Enhancement (methods)
• Image Interpretation, Computer-Assisted (methods)
• Liver (diagnostic imaging)
• Rats
• Rats, Sprague-Dawley
• Reproducibility of Results
• Sensitivity and Specificity
• Species Specificity
• Ultrasonography (methods)