Comparative photodynamic therapy cytotoxicity of mannose-conjugated chlorin and talaporfin sodium in cultured human and rat cells.

Abstract

Photodynamic therapy (PDT) is a Food and Drug Administration authorized method for cancer treatment, which uses photosensitizer and laser photo-irradiation to generate reactive oxygen species to induce cell death in tumors. Photosensitizers have been progressively developed, from first to third generation, with improvements in cell specificity, reduced side effects and toxicity, increased sensitivity for irradiation and reduced persistence of photosensitizer in healthy cells. These improvements have been achieved by basic comparative experiments between current and novel photosensitizers using cell lines; however, photosensitizers should be carefully evaluated because they may have cell type specificity. In the present study, we compared a third-generation photosensitizer, β-mannose-conjugated chlorin (β-M-chlorin), with the second generation, talaporfin sodium (NPe6), using seven different rat and human cell lines and a neuronal/glial primary culture prepared from rat embryos. NPe6 was more effective than β-M-chlorin in human-derived cell lines, and β-M-chlorin was more effective than NPe6 in rat primary cultures and rat-derived cell lines, except for the rat pheochromocytoma cell line, PC12. These differences of phototoxicity in different cell types are not because of differences in photosensitivity between the photosensitizers, but rather are associated with different distribution and accumulation rates in the different cell types. These data suggest that evaluation of photosensitizers for PDT should be carried out using as large a variety of cell types as possible because each photosensitizer may have cell type specificity.

• Authors: Yo Shinoda, Tsutomu Takahashi, Jiro Akimoto, Megumi Ichikawa, Hiromi Yamazaki, Atsushi Narumi, Shigenobu Yano, Yasuyuki Fujiwara
• Journal: The Journal of toxicological sciences (J Toxicol Sci) Vol. 42 Issue 1 Pg. 111-119 ( 2017) ISSN: 1880-3989 [Electronic] Japan
• PMID: 28070104 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antineoplastic Agents
• Photosensitizing Agents
• Porphyrins
• chlorin
• Talaporfin
• Mannose

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Cell Line
• Cell Line, Tumor
• Cell Survival (drug effects, radiation effects)
• Cells, Cultured
• Humans
• Light
• Mannose (chemistry, pharmacology)
• Neurons (drug effects, metabolism, radiation effects)
• PC12 Cells
• Photochemotherapy
• Photosensitizing Agents (pharmacology)
• Porphyrins (chemistry, pharmacology)
• Rats
• Rats, Wistar