Linking expression of aquaporin 3 to activation of JNK pathway.

Abstract	Aquaporin 3 (AQP3) has been shown to be low in the amnion and chorion tissues of patients with oligohydramnios and that S. miltiorrhiza, a Chinese herbal medicine, results in increased AQP3 in human amniotic epithelial cells (hAECs). Here, we provide evidence for the involvement of the JNK pathway in AQP3 regulation in isolated oligohydramnios tissues in vitro, in hAECs derived from normal amniotic fluid and fluid from patients with isolated oligohydramnios. Phosphorylation of JNK was suppressed by pretreatment of cells with JNK-specific inhibitor (SP600125) and was up-regulated by S. miltiorrhiza; S. miltiorrhiza combined with SP600125 prevented SP600125-induced down-regulation of phospho-JNK both in normal amniotic fluid volume and in isolated oligohydramnios. In isolated oligohydramnios, AQP3 expression was signiﬁcantly suppressed by SP600125 in a concentration- and time-dependent mannner, while its expression was up-regulated by S. miltiorrhiza. S. miltiorrhiza combined with SP600125 inhibited the increased expression of AQP3 relative to the S. miltiorrhiza treated group. Together, the data suggest that c-jun N-terminal kinase (JNK) pathway unerlies the regulation of AQP3 by S. miltiorrhiza amnion and chorion tissues.
Authors	Qi Shen, Jingjing Wang, Qingfeng Zhou, Zhaojun Shen, Hui Luo, Xuejiao Tao, Xueqiong Zhu
Journal	Frontiers in bioscience (Landmark edition) (Front Biosci (Landmark Ed)) Vol. 22 Issue 2 Pg. 258-267 (01 01 2017) ISSN: 2768-6698 [Electronic] Singapore
PMID	27814615 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	AQP3 protein, human Anthracenes Drugs, Chinese Herbal Aquaporin 3 pyrazolanthrone
Topics	Adult Amnion (drug effects, metabolism) Amniotic Fluid (drug effects) Anthracenes (administration & dosage) Aquaporin 3 (metabolism) Case-Control Studies Cells, Cultured Drugs, Chinese Herbal (administration & dosage) Epithelial Cells (drug effects, metabolism) Female Humans MAP Kinase Signaling System (drug effects) Oligohydramnios (drug therapy, metabolism) Pregnancy Salvia miltiorrhiza Young Adult

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