Abstract |
Ribosome biogenesis is the process of synthesis of the cellular ribosomes which mediate protein translation. Integral with the ribosomes are four cytoplasmic ribosomal RNAs (rRNAs) which show extensive post-transcriptional modifications including 2'-O-methylation and pseudouridylation. Several hereditary hematologic diseases including Diamond-Blackfan anemia have been shown to be associated with defects in ribosome biogenesis. Thalassemia is the most important hematologic inherited genetic disease worldwide, and this study examined the post-transcriptional ribose methylation status of three specific active sites of the 28S rRNA molecule at positions 1858, 4197 and 4506 of β- thalassemia trait carriers and normal controls. Samples from whole blood and cultured erythroid cells were examined. Results showed that site 4506 was hypermethylated in β- thalassemia trait carriers in both cohorts. Expression of fibrillarin, the ribosomal RNA methyltransferase as well as snoRNAs were additionally quantified by RT-qPCR and evidence of dysregulation was seen. Hemoglobin E trait carriers also showed evidence of dysregulation. These results provide the first evidence that ribosome biogenesis is dysregulated in β- thalassemia trait carriers.
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Authors | Wannapa Sornjai, Pathrapol Lithanatudom, Jenny Erales, Philippe Joly, Alain Francina, Sabine Hacot, Suthat Fucharoen, Saovaros Svasti, Jean Jacques Diaz, Hichem C Mertani, Duncan R Smith |
Journal | International journal of biological macromolecules
(Int J Biol Macromol)
Vol. 94
Issue Pt A
Pg. 728-734
(Jan 2017)
ISSN: 1879-0003 [Electronic] Netherlands |
PMID | 27765567
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier B.V. All rights reserved. |
Chemical References |
- Chromosomal Proteins, Non-Histone
- RNA, Ribosomal, 28S
- RNA, Small Nucleolar
- fibrillarin
- pseudouridylic acid
- Hemoglobin E
- Uridine Monophosphate
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Topics |
- Case-Control Studies
- Chromosomal Proteins, Non-Histone
(genetics, metabolism)
- Gene Expression
- Hematopoietic Stem Cells
(metabolism, pathology)
- Hemoglobin E
(genetics, metabolism)
- Heterozygote
- Humans
- Leukocytes, Mononuclear
(metabolism, pathology)
- Methylation
- Primary Cell Culture
- Protein Biosynthesis
- RNA Processing, Post-Transcriptional
- RNA, Ribosomal, 28S
(genetics, metabolism)
- RNA, Small Nucleolar
(genetics, metabolism)
- Ribosomes
(genetics, metabolism)
- Uridine Monophosphate
(genetics, metabolism)
- beta-Thalassemia
(genetics, metabolism, pathology)
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