A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry.

Abstract

In this study, we demonstrated an oxidative method with free radical to generate 3,5,4'-trihydroxy-trans-stilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI-MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4'-dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of its in situ generation of oxidative derivatives followed by LC-ESI-MS/MS can be utilized to evaluate different metabolites in various conditions.

• Authors: Wangta Liu, Yow-Ling Shiue, Yi-Reng Lin, Hugo You-Hsien Lin, Shih-Shin Liang
• Journal: Current analytical chemistry (Curr Anal Chem) Vol. 11 Issue 4 Pg. 300-306 (Oct 2015) ISSN: 1573-4110 [Print] United Arab Emirates
• PMID: 27594817 (Publication Type: Journal Article)