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In vitro and in vivo antibiofilm potential of 2,4-Di-tert-butylphenol from seaweed surface associated bacterium Bacillus subtilis against group A streptococcus.

Abstract
Biofilm formation of Group A Streptococcus (GAS) is recognized as an important virulent determinant. The present study reports the antibiofilm potential of seaweed (Gracilaria gracilis) surface associated Bacillus subtilis against GAS. Purification revealed 2,4-Di-tert-butyl-phenol (DTBP) as the active principle. DTBP exhibited a dose dependent antibiofilm activity against GAS (SF370 & six different clinical M serotypes). Microscopic analysis revealed changes in cell surface architecture and reduced thickness upon DTBP treatment. Results of extracellular polymeric substance quantification, microbial adhesion to hydrocarbon assay and fourier transform infrared spectroscopic analysis suggested that DTBP probably interferes with the initial adhesion stage of biofilm formation cascade. Reduction in hyaluronic acid synthesis goes in unison with blood survival assay wherein, increased susceptibility to phagocytosis was observed. In vivo studies using Caenorhabditis elegans manifested the reduction in adherence and virulence, which prompts further investigation of the potential of DTBP for the treatment of GAS infections.
AuthorsDharmaprakash Viszwapriya, Udayakumar Prithika, Sundaresan Deebika, Krishnaswamy Balamurugan, Shunmugiah Karutha Pandian
JournalMicrobiological research (Microbiol Res) Vol. 191 Pg. 19-31 (Oct 2016) ISSN: 1618-0623 [Electronic] Germany
PMID27524650 (Publication Type: Journal Article)
CopyrightCopyright © 2016 Elsevier GmbH. All rights reserved.
Chemical References
  • Anti-Bacterial Agents
  • Biopolymers
  • Phenols
  • 2,4-di-tert-butylphenol
Topics
  • Animals
  • Anti-Bacterial Agents (isolation & purification, pharmacology)
  • Bacillus subtilis (chemistry, isolation & purification)
  • Bacterial Adhesion (drug effects)
  • Biofilms (drug effects, growth & development)
  • Biopolymers (analysis)
  • Caenorhabditis elegans (microbiology)
  • Disease Models, Animal
  • Microscopy
  • Phagocytosis
  • Phenols (isolation & purification, pharmacology)
  • Seaweed (microbiology)
  • Spectroscopy, Fourier Transform Infrared
  • Streptococcal Infections (microbiology, pathology)
  • Streptococcus pyogenes (cytology, drug effects, physiology)
  • Virulence

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