microRNA-214 Governs Lung Cancer Growth and Metastasis by Targeting Carboxypeptidase-D.
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| Abstract |
Lung cancer is one of the most malignant cancers with a high metastatic potential. The purpose of this study was to study the role and the underlying mechanism of miR-214 in lung cancer progression. The expression of miR-214 in normal lung and lung cancer tissue was analyzed by quantitative real-time PCR analysis. Furthermore, H1299 cells were infected with miR-214 lentivirus, and the effect of infection on cell viability and migration was analyzed. Carboxypeptidase-D (CPD), as a potential target of miR-214, was characterized in either normal lung or lung cancer tissues. The interaction of CPD expression with the tumor suppressing effect of miR-214 was characterized. We demonstrated that low miR-214 expression is a hallmark of lung cancer, especially high-grade and metastatic cancer. In vitro studies in H1299 cells confirmed that low miR-214 expression is associated with enhanced proliferation and migratory abilities. Similarly, CPD overexpression coincides with high-grade lung cancer and the CPD overexpression could reverse the inhibitory effects of miR-214. miR-214 is a tumor suppressor in lung cancer. miR-214 inhibits lung cancer progression by targeting CPD. The miR-214-CPD axis may be a therapeutic axis for lung cancer patients.
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| Authors | Xiaojian Zhao, Caiping Lu, Weiwei Chu, Yaxiao Zhang, Bing Zhang, Qiang Zeng, Renfeng Wang, Zhe Li, Baolei Lv, Jiabao Liu |
| Journal | DNA and cell biology (DNA Cell Biol) Vol. 35 Issue 11 Pg. 715-721 (Nov 2016) ISSN: 1557-7430 [Electronic] United States |
| PMID | 27494742 (Publication Type: Journal Article) |
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