Dual CD44 and folate receptor-targeted nanoparticles for cancer diagnosis and anticancer drug delivery.

Abstract

Dual CD44 and folate receptor targetable nanoparticles (NPs) based on hyaluronic acid-ceramide-folic acid (HACE-FA) were fabricated for improving tumor targetability. HACE-FA was synthesized via esterification between the carboxylic group of FA and hydroxyl group of HA. Doxorubicin (DOX)-loaded HACE-FA NPs, with a mean diameter of 120-130nm, narrow size distribution, and negative zeta potential, were prepared. The drug release from HACE-FA NPs were significantly increased in acidic pH (pH5.5) compared with physiological pH (7.4) (p<0.05). The cellular accumulation of the drug in HACE-FA NPs group was higher than that of HACE NPs group in SKOV-3 cells (human ovarian cancer cells; CD44 and folate receptor (FR)-positive cells). Dual targetability of HACE-FA NPs, compared to HACE NPs, was also verified in the SKOV-3 tumor-xenografted mouse model by near-infrared fluorescence (NIRF) imaging. Twenty-four hours after injection, HACE-FA NPs were accumulated mainly in tumor regions and their fluorescence intensity was 4.82-fold higher than that of HACE NPs (p<0.05). These findings suggest successful application of HACE-FA NPs for the accurate delivery of anticancer drugs to ovarian cancer.

• Authors: Jae-Young Lee, Ubonvan Termsarasab, Ju-Hwan Park, Song Yi Lee, Seung-Hak Ko, Jae-Seong Shim, Suk-Jae Chung, Hyun-Jong Cho, Dae-Duk Kim
• Journal: Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 236 Pg. 38-46 (08 28 2016) ISSN: 1873-4995 [Electronic] Netherlands
• PMID: 27320169 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• Ceramides
• Drug Carriers
• Folate Receptors, GPI-Anchored
• Hyaluronan Receptors
• Doxorubicin
• Hyaluronic Acid
• Folic Acid

Topics

• Animals
• Antineoplastic Agents (administration & dosage)
• Cell Line, Tumor
• Ceramides (chemistry)
• Doxorubicin (administration & dosage)
• Drug Carriers
• Drug Liberation
• Female
• Folate Receptors, GPI-Anchored (metabolism)
• Folic Acid (chemistry)
• Humans
• Hyaluronan Receptors (metabolism)
• Hyaluronic Acid (chemistry)
• Mice
• Molecular Targeted Therapy
• Nanoparticles (chemistry)
• Optical Imaging
• Ovarian Neoplasms (diagnosis, drug therapy)
• Particle Size