Abstract | BACKGROUND: MATERIALS AND METHODS: RESULTS: HCT-116-GFP-RFP cells subsequently formed primary tumors in the spleen, as well as metastatic colonies in the liver and retroperitoneum by 28 days after cell transplantation. Using an Olympus FV1000 confocal microscope, it was possible to clearly image mitosis of the dual-colored colon cancer cells in the primary tumor as well as liver and other metastases. Multi-nucleate cancer cells, in addition to mono-nucleate cancer cells and their mitosis, were observed in the primary tumor and metastasis. Multi-nucleate HCT-116-GFP-RFP cells were also observed after culture of the primary and metastatic tumors. A similar ratio of mono-nucleate, multi-nucleate, and mitotic cells grew from the primary and metastatic tumors in culture, suggesting similarity of the nuclear-cytoplasmic dynamics of primary and metastatic cancer cells, further emphasizing the stochastic nature of metastasis. CONCLUSION: Our results demonstrate a similar heterogeneity of nuclear-cytoplasmic dynamics within primary tumors and metastases, which may be an important factor in the stochastic nature of metastasis.
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Authors | Kosuke Hasegawa, Atsushi Suetsugu, Miki Nakamura, Takuro Matsumoto, Hitomi Aoki, Takahiro Kunisada, Michael Bouvet, Masahito Shimizu, Robert M Hoffman |
Journal | Anticancer research
(Anticancer Res)
Vol. 36
Issue 5
Pg. 2113-7
(May 2016)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 27127111
(Publication Type: Journal Article)
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Copyright | Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
Topics |
- Animals
- Cell Nucleus
(pathology)
- Colonic Neoplasms
(pathology)
- Cytoplasm
(pathology)
- HCT116 Cells
- Humans
- Mice
- Mice, Nude
- Neoplasm Metastasis
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