Sepsis results in an altered renal metabolic and osmolyte profile.

Abstract

BACKGROUND:

Sepsis remains a major health-care burden and source of morbidity and mortality. Acute kidney injury and failure frequently accompanies severe sepsis and contributes to this burden. Despite a great deal of research, the exact mechanisms underlying renal failure in sepsis are poorly understood. This study aims to further understand metabolic changes in renal tissue during sepsis.

MATERIALS AND METHODS:

Experimental sepsis was induced by cecal ligation and puncture (CLP) in C57BL/6 mice. Serum and organs were harvested 8 h after CLP. Markers of renal function including serum creatinine, blood urea nitrogen, and cystatin C were measured. Whole kidneys were analyzed for a global biochemical profile via liquid chromatography/tandem mass spectrometry by Metabolon.

RESULTS:

CLP induced renal injury as evidenced by elevated serum creatinine, blood urea nitrogen, and cystatin C. Global energetic profile in sepsis showed an increase in glycolytic intermediates with decreased flux through the tricarboxylic acid (TCA) cycle. Multiple inflammatory markers were elevated in response to CLP. Levels of osmotic regulators varied, with an overall increase in pinitol, urea, and taurine in response to CLP.

CONCLUSIONS:

CLP resulted in dramatic changes in the renal macromolecular milieu. There appears to be an increased dependence on glycolysis and diminished flush through the TCA cycle. In addition, changes in renal osmolytes including pinitol, urea, and taurine were observed, perhaps uncovering an additional change with implications on renal function during sepsis.

• Authors: Paul Waltz, Evie Carchman, Hernando Gomez, Brian Zuckerbraun
• Journal: The Journal of surgical research (J Surg Res) Vol. 202 Issue 1 Pg. 8-12 (May 01 2016) ISSN: 1095-8673 [Electronic] United States
• PMID: 27083942 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Published by Elsevier Inc.

Chemical References

• Biomarkers
• Cystatin C
• Creatinine

Topics

• Acute Kidney Injury (diagnosis, etiology, metabolism)
• Animals
• Biomarkers (metabolism)
• Blood Urea Nitrogen
• Creatinine (blood)
• Cystatin C (blood)
• Kidney (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Sepsis (complications, metabolism)