O-Specific Polysaccharide-Specific Memory B Cell Responses in Young Children, Older Children, and Adults Infected with Vibrio cholerae O1 Ogawa in Bangladesh.

Abstract

Cholera caused by Vibrio cholerae O1 confers at least 3 to 10 years of protection against subsequent disease regardless of age, despite a relatively rapid fall in antibody levels in peripheral blood, suggesting that memory B cell responses may play an important role in protection. The V. cholerae O1-specific polysaccharide (OSP) component of lipopolysaccharide (LPS) is responsible for serogroup specificity, and it is unclear if young children are capable of developing memory B cell responses against OSP, a T cell-independent antigen, following cholera. To address this, we assessed OSP-specific memory B cell responses in young children (2 to 5 years, n = 11), older children (6 to 17 years, n = 21), and adults (18 to 55 years, n = 28) with cholera caused by V. cholerae O1 in Dhaka, Bangladesh. We also assessed memory B cell responses against LPS and vibriocidal responses, and plasma antibody responses against OSP, LPS, and cholera toxin B subunit (CtxB; a T cell-dependent antigen) on days 2 and 7, as well as days 30, 90, and 180 after convalescence. In all age cohorts, vibriocidal responses and plasma OSP, LPS, and CtxB-specific responses peaked on day 7 and fell toward baseline over the follow-up period. In comparison, we were able to detect OSP memory B cell responses in all age cohorts of patients with detectable responses over baseline for 90 to 180 days. Our results suggest that OSP-specific memory B cell responses can occur following cholera, even in the youngest children, and may explain in part the age-independent induction of long-term immunity following naturally acquired disease.

• Authors: Amena Aktar, M Arifur Rahman, Sadia Afrin, M Omar Faruk, Taher Uddin, Aklima Akter, M Israk Nur Sami, Tahirah Yasmin, Fahima Chowdhury, Ashraful I Khan, Daniel T Leung, Regina C LaRocque, Richelle C Charles, Taufiqur Rahman Bhuiyan, Anjali Mandlik, Meagan Kelly, Pavol Kováč, Peng Xu, Stephen B Calderwood, Jason B Harris, Firdausi Qadri, Edward T Ryan
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 23 Issue 5 Pg. 427-435 (05 2016) ISSN: 1556-679X [Electronic] United States
• PMID: 27009211 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Chemical References

• Antibodies, Bacterial
• Immunoglobulin A
• Immunoglobulin M
• Lipopolysaccharides
• O Antigens
• Cholera Toxin

Topics

• Adolescent
• Adult
• Antibodies, Bacterial (blood)
• B-Lymphocytes (immunology)
• Bangladesh (epidemiology)
• Child
• Child, Preschool
• Cholera (epidemiology, immunology, microbiology)
• Cholera Toxin (immunology)
• Female
• Humans
• Immunoglobulin A (blood)
• Immunoglobulin M (blood)
• Immunologic Memory
• Lipopolysaccharides (immunology)
• Male
• Middle Aged
• O Antigens (immunology)
• Vibrio cholerae O1 (chemistry, immunology)
• Young Adult