Chromatin accessibility is tightly regulated by multiple factors/mechanisms to establish different cell type-specific gene expression programs from a single genome. Dysregulation of this process can lead to diseases including
cancer. The Mi-2/
nucleosome remodeling and deacetylase (
NuRD) complex is thought to orchestrate
chromatin structure using its intrinsic
nucleosome remodeling and
histone deacetylase activities. However, the detailed mechanisms by which the
NuRD complex regulates
chromatin structure in vivo are not yet known. To explore the regulatory mechanisms of the
NuRD complex, we mapped genome-wide localization of MBD3, a structural component of NuRD, in a human
breast cancer cell line (MDA-MB-231) using a modified ChIP-seq protocol. Our data showed high quality localization information (i.e., high mapping efficiency and low PCR duplication rate) and excellent consistency between
biological replicates. The data are deposited in the Gene Expression Omnibus (GSE76116).