Effect of turmeric on colon histology, body weight, ulcer, IL-23, MPO and glutathione in acetic-acid-induced inflammatory bowel disease in rats.

Abstract

BACKGROUND:

This study investigates the protective effects of turmeric (Curcuma longa, CL) on acetic acid-induced colitis in rats.

METHOD:

Inflammatory bowel disease (IBD) was induced in male Wistar rats by intra-rectal administration of 1 ml of 4% acetic acid at 8 cm proximal to the anus for 30 s. Curcuma longa (CL) powder, (1, 10, or 100 mg/kg/day) was administered for either 3 days before or after IBD for 7 days. The body weight, macroscopic and microscopic analysis of the colon of CL-treated IBD rats and that of control rats (no IBD, no CL) were performed on 0 day, 2, 4 and 7th day. Myeloperoxidase (MPO), IL-23 and glutathione levels in control, untreated and treated rats were measured by ELISA.

RESULTS:

CL significantly (P < 0.05) improved IBD-induced reduction in mean body weight and mean macroscopic ulcer score. Administration of CL also significantly (P < 0.01) reduced the mean microscopic ulcer score when compared to untreated IBD control. Intake of CL by rats resulted in a significant (P < 0.05) increase in the mean serum glutathione level compared to untreated control. CL reduced both MPO and IL-23 levels in the colonic mucosa of the rat.

CONCLUSION:

CL improved body weight gain, mean macroscopic and microscopic ulcer scores in the colon of rats suffering from acetic acid-induced IBD. CL reduced both MPO and IL-23 in the mucosa of the colon. The increase in the mean serum glutathione level may help in the reduction of oxidative stress associated with IBD.

• Authors: Salim M A Bastaki, Mohammed Majed Al Ahmed, Ahmed Al Zaabi, Naheed Amir, Ernest Adeghate
• Journal: BMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 16 Pg. 72 (Feb 23 2016) ISSN: 1472-6882 [Electronic] England
• PMID: 26907175 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Interleukin-23
• Plant Extracts
• Peroxidase
• Glutathione
• Acetic Acid

Topics

• Acetic Acid
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Antioxidants (pharmacology, therapeutic use)
• Body Weight (drug effects)
• Colitis, Ulcerative (chemically induced, drug therapy, metabolism, pathology)
• Colon (drug effects, metabolism, pathology)
• Curcuma
• Glutathione (metabolism)
• Inflammatory Bowel Diseases (drug therapy, metabolism, pathology)
• Interleukin-23 (blood)
• Intestinal Mucosa (drug effects, metabolism, pathology)
• Male
• Oxidative Stress (drug effects)
• Peroxidase (blood)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Rats, Wistar
• Ulcer