Smart Cancer Cell Targeting Imaging and Drug Delivery System by Systematically Engineering Periodic Mesoporous Organosilica Nanoparticles.

Abstract

The integration of diagnosis and therapy into one nanoplatform, known as theranostics, has attracted increasing attention in the biomedical areas. Herein, we first present a cancer cell targeting imaging and drug delivery system based on engineered thioether-bridged periodic mesoporous organosilica nanoparticles (PMOs). The PMOs are stably and selectively conjugated with near-infrared fluorescence (NIRF) dye Cyanine 5.5 (Cy5.5) and anti-Her2 affibody on the outer surfaces to endow them with excellent NIRF imaging and cancer targeting properties. Also, taking the advantage of the thioether-group-incorporated mesopores, the release of chemotherapy drug doxorubicin (DOX) loaded in the PMOs is responsive to the tumor-related molecule glutathione (GSH). The drug release percentage reaches 84.8% in 10 mM of GSH solution within 24 h, which is more than 2-fold higher than that without GSH. In addition, the drug release also exhibits pH-responsive, which reaches 53.6% at pH 5 and 31.7% at pH 7.4 within 24 h. Confocal laser scanning microscopy and flow cytometry analysis demonstrate that the PMOs-based theranostic platforms can efficiently target to and enter Her2 positive tumor cells. Thus, the smart imaging and drug delivery nanoplatforms induce high tumor cell growth inhibition. Meanwhile, the Cy5.5 conjugated PMOs perform great NIRF imaging ability, which could monitor the intracellular distribution, delivery and release of the chemotherapy drug. In addition, cell viability and histological assessments show the engineered PMOs have good biocompatibility, further encouraging the following biomedical applications. Over all, the systemically engineered PMOs can serve as a novel cancer cell targeting imaging and drug delivery platform with NIRF imaging, GSH and pH dual-responsive drug release, and high tumor cell targeting ability.

• Authors: Nan Lu, Ying Tian, Wei Tian, Peng Huang, Ying Liu, Yuxia Tang, Chunyan Wang, Shouju Wang, Yunyan Su, Yunlei Zhang, Jing Pan, Zhaogang Teng, Guangming Lu
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 8 Issue 5 Pg. 2985-93 (Feb 10 2016) ISSN: 1944-8252 [Electronic] United States
• PMID: 26767305 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Organosilicon Compounds
• Sulfides
• Doxorubicin

Topics

• Antineoplastic Agents (administration & dosage, chemistry)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Cell Tracking (methods)
• Doxorubicin (administration & dosage, chemistry)
• Drug Delivery Systems
• Humans
• Microscopy, Confocal
• Molecular Imaging (methods)
• Nanoparticles (chemistry, therapeutic use)
• Neoplasms (drug therapy, pathology)
• Organosilicon Compounds (chemistry)
• Porosity
• Sulfides (chemistry)
• Theranostic Nanomedicine